Anti-ARL4A Rabbit Polyclonal Antibody for IHC-P, IF/ICC, ELISA - P40617

Core Product Specifications and Parameters

Biological Background: ARL4A Function and Localization

• ARL4A (ADP-ribosylation factor-like protein 4A), also known as ARL4, is a member of the ARF-like (ARL) family of small GTP-binding proteins.
• It cycles between an inactive GDP-bound state and an active GTP-bound state, with the exchange and hydrolysis rates regulated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs).
• Unlike some ARF proteins, ARL4A does not function as an allosteric activator of the cholera toxin catalytic subunit.
• In its GDP-bound form, ARL4A recruits cytohesin family members (CYTH1, CYTH2, CYTH3, CYTH4) to the plasma membrane, facilitating membrane dynamics.
• Subcellular localization is dynamic and includes the cell membrane, cytoplasm, nucleus, and nucleolus, reflecting its role in diverse cellular compartments.
• ARL4A is a lipoprotein that undergoes myristoylation, a modification that likely mediates its membrane association.
• The protein is identified as part of the human reference proteome and has been detected in high-throughput proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 121–200 within the C-terminal region of human ARL4A, a domain relevant for binding partners; antibody detection may be independent of the nucleotide-bound state.
• For IHC-P and IF/ICC applications, consider antigen retrieval and permeabilization protocols appropriate for membrane and nuclear targets.
• For ELISA, cross-validate with full-length recombinant ARL4A protein to confirm binding specificity and optimize coating conditions.
• The antibody shows cross-reactivity with mouse and rat ARL4A, but sequence alignment is recommended to confirm epitope conservation before use in non-human species.
• As a polyclonal antibody, lot-to-lot consistency should be validated in the end-user’s assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and research institutions with a comprehensive portfolio of IVD raw materials, including this anti-ARL4A polyclonal antibody for studying GTPase signaling and membrane trafficking. As a single procurement bridge, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—streamlining your path from concept to clinic. Our flexible supply model supports both established and emerging diagnostic assays with technical consultation and reliable delivery.