Anti-ARL13B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q3SXY8

Core Product Specifications and Parameters

Biological Background: ARL13B Function and Localization

• ARL13B (ADP-ribosylation factor-like protein 13B), also known as ARL2L1 or JBTS8, is a cilium-specific GTPase belonging to the ARL family.
• It is essential for controlling the microtubule-based axoneme structure of primary cilia, likely by maintaining the association between intraflagellar transport (IFT) subcomplexes A and B.
• ARL13B binds GTP but its GTPase activity remains unclear; it is involved in ciliary trafficking of INPP5E, a phosphatidylinositol phosphatase critical for ciliogenesis (PubMed:38219074). Related references: PMID:38219074
• The protein plays a crucial role in neural tube patterning and cerebral cortex development by regulating ciliary signaling during radial glial scaffold formation and interneuron migration.
• ARL13B may also regulate ARF6- and RAB22A-dependent endocytic recycling traffic, linking it to membrane trafficking pathways (PubMed:23223633). Related references: PMID:23223633
• Subcellularly, it localizes to the cilium membrane, ciliary axoneme, cell membrane, cytoplasm, and cytoskeleton.
• It is expressed in the developing brain, consistent with its role in neurodevelopment.
• Mutations in ARL13B are associated with Joubert syndrome (JBTS8), a ciliopathy characterized by brain malformations.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1-240 of human ARL13B; therefore, the antibody may detect full-length protein and potential N-terminal cleavage products.
• For Western blotting, a band around 49 kDa is expected; endogenous expression may vary, so validate using positive control lysates (e.g., ciliated cell lines).
• In immunofluorescence, ARL13B localizes to cilia; use appropriate fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) to visualize ciliary staining.
• Due to the polyclonal nature, lot-to-lot consistency should be verified for critical quantitative assays.
• The antibody is suitable for ELISA; consider pairing with a monoclonal detection antibody if developing a sandwich immunoassay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic manufacturers and researchers by providing validated antibodies, bulk ancillary reagents, and rare-target raw materials. For ciliopathy and neuroscience research, we offer reliable anti-ARL13B antibodies alongside optimized secondary detection systems. Contact us to explore customized sourcing solutions spanning from early-stage R&D to clinical assay development.