Anti-ARL1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P40616

Core Product Specifications and Parameters

Biological Background: ARL1 Function and Localization

• ARL1 (ADP-ribosylation factor-like protein 1) is a small GTPase of the Arf family, acting as a molecular switch in membrane trafficking and organelle dynamics.
• It functions primarily at the trans-Golgi network, recruiting effectors such as golgins, arfaptins, and Arf-GEFs to regulate Golgi structure and protein transport. Related references: PMID:21239483 PMID:22679020 PMID:27373159
• Through these interactions, ARL1 modulates fundamental cellular processes including cell polarity, innate immunity, and regulated protein secretion. Related references: PMID:22981988
• In pancreatic beta cells, the ARL1-arfaptin complex is specifically implicated in maintaining insulin secretion. Related references: PMID:22981988
• ARL1 is anchored to the Golgi apparatus and trans-Golgi network membranes via N-terminal myristoylation.
• It is broadly expressed, detected at the protein level in heart, liver, lung, and various fetal and adult tissues.
• Key structural and functional features include GTP-binding, magnesium-dependent activity, lipoprotein modification, and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a fragment within the GTPase domain, which is highly conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For western blotting, an expected band of approximately 20 kDa should be detected; consider optimizing blocking and antibody dilution for your samples.
• In immunofluorescence/ICC, a typical Golgi-like perinuclear staining pattern is anticipated; fixation and permeabilization conditions should be validated for the cell line used.
• For ELISA applications, standard indirect or sandwich ELISA protocols can be tested; the antibody may be used as a capture or detection reagent after appropriate coating and blocking optimization.
• Always include appropriate positive and negative controls and confirm antibody performance in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated sourcing partner for IVD raw materials, offering a comprehensive portfolio of monoclonal and polyclonal antibodies, validated antibody pairs, and ancillary reagents. For researchers focusing on Golgi biology and membrane trafficking, this anti-ARL1 product provides a reliable tool to expedite assay development. Our supply chain expertise ensures consistent quality and availability—from bulk reagent orders to customized rare-target sourcing—supporting your path from concept to clinic.