Anti-ARIH1 Rabbit Polyclonal Antibody for WB and ELISA - Q9Y4X5

Core Product Specifications and Parameters

Biological Background: ARIH1 Function and Localization

• ARIH1 (also known as HHARI, ARI-1, UbcH7-binding protein, MOP-6) is an E3 ubiquitin-protein ligase.
• It catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. Related references: PMID:15236971, PMID:21532592, PMID:23707686
• Acts as an atypical E3 ligase by associating with cullin-RING ubiquitin ligase (CRL) complexes, mediating the initial ubiquitin on CRL substrates, which is elongated by CDC34/UBE2R1 and UBE2R2. Related references: PMID:27565346
• Ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes. Related references: PMID:24076655, PMID:27565346
• Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2; reported outcomes include promotion of cap-binding and translation arrest or degradation. Also mediates ISGylation of EIF4E2. Related references: PMID:25624349, PMID:14623119, PMID:17289916
• In vitro, controls degradation of SUN2, a component of the LINC complex, potentially influencing nuclear morphology and localization. Related references: PMID:29689197
• Contains zinc-finger domains and is involved in the ubiquitin conjugation pathway as a metal-binding transferase.
• Widely expressed across human tissues, with localization to the cytoplasm, nucleus, and Cajal bodies.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the C-terminal region (amino acids 400–557) of human ARIH1. This region shows high sequence homology across human, mouse, and rat, consistent with the reported cross-reactivity.
• For Western blotting, ARIH1 has a predicted molecular weight of ~64 kDa. Use standard whole-cell or tissue lysates; validate the band size in your system, as post-translational modifications may cause shifts.
• As a polyclonal antibody, ensure consistent lot-to-lot performance by including appropriate positive and negative controls in each experiment.
• For ELISA, consider direct antigen coating or sandwich formats, optimizing antibody dilution and secondary detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to IVD raw materials, from antibodies to ancillary reagents. For ubiquitin pathway targets like ARIH1, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk reagent supply to support your assay development. Our expert team also assists in rare-target raw-material sourcing and technical optimization.