Anti-ARID5A Mouse Monoclonal Antibody for WB, ELISA - Q03989

Core Product Specifications and Parameters

Biological Background: ARID5A Function and Localization

• ARID5A (AT-rich interactive domain-containing protein 5A), also known as modulator recognition factor 1 (MRF-1), is a DNA- and RNA-binding protein implicated in transcription regulation and mRNA stability.
• As a DNA-binding protein, it may regulate transcription and act as a repressor by binding to AT-rich stretches in the promoter region of target genes (PubMed:8649988). Related references: PMID:8649988
• ARID5A can repress ESR1-mediated transcriptional activation, acting as a corepressor for selective nuclear hormone receptors (PubMed:15941852). Related references: PMID:15941852
• As an RNA-binding protein, it stabilizes selective inflammation-related mRNAs, such as IL6, STAT3, and TBX21, by binding to stem-loop structures in their 3'UTRs and preventing degradation by ZC3H12A and RC3H1. It contributes to elevated IL6 levels possibly implicated in autoimmunity (PubMed:32209697 for IL6 stabilization). Related references: PMID:32209697
• In CD4+ T-cells, ARID5A influences Th17 and Th1 cell differentiation by stabilizing STAT3 and TBX21 mRNAs, respectively, and may play a role in septic shock establishment (By similarity).
• It may positively regulate chondrocyte-specific transcription (e.g., COL2A1) in collaboration with SOX9 and influence chondrocyte differentiation, stimulating early-stage and inhibiting later-stage differentiation (By similarity).
• Subcellular localization: Predominantly nuclear.
• Keywords: DNA-binding, RNA-binding, transcription regulation, repressor, activator, innate immunity, phosphorylation, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 1-594 of human ARID5A (full-length), suggesting the antibody may recognize multiple epitopes on the denatured protein. Consider using it for Western blot (WB) detection of ARID5A in human and mouse samples (predicted MW ~64 kDa).
• For ELISA applications, validate the antibody’s binding to the native protein or relevant peptide fragments in your specific assay format.
• Note cross-reactivity with mouse ARID5A; verify performance in mouse-derived samples if intended for cross-species applications.
• As a monoclonal antibody, it offers consistent lot-to-lot performance; however, we recommend performing routine positive and negative controls.
• Store as shipped on ice bags and aliquot to avoid freeze-thaw cycles upon arrival.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering a broad portfolio including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For researchers and diagnostic developers targeting ARID5A in transcriptional regulation or inflammation, we provide reliable access to this antibody and can assist with rare target raw-material procurement. From concept to clinic, CamelBio supports your diagnostic development with technical and supply chain expertise.