Anti-ARID2 Polyclonal Antibody for WB, IP, ELISA - Q68CP9

Core Product Specifications and Parameters

Biological Background: ARID2 Function and Localization

• ARID2 (AT-rich interactive domain-containing protein 2), also known as BAF200, is a core subunit of the PBAF (SWI/SNF-B) chromatin remodeling complex.
• It facilitates transcriptional activation and repression of specific genes by altering DNA-nucleosome topology.
• ARID2 is essential for the structural stability of the PBAF complex.
• It may participate in directing the PBAF complex to distinct genomic loci.
• The protein contains a zinc-finger domain and is subject to post-translational modifications including phosphorylation and acetylation.
• ARID2 localizes to the nucleus and is highly expressed in the heart, suggesting a role in cardiac gene regulation.
• It is associated with diseases such as intellectual disability when mutated.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1586-1835 of human ARID2. For Western blot, expect a band near 197 kDa. Use appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In IP applications, consider the polyclonal nature and validate capture efficiency in the relevant cell or tissue lysate.
• For ELISA, optimize coating antigen and antibody dilution using a standard curve with recombinant ARID2 protein or peptide.
• Since cross-reactivity is reported for human, verify specificity in human-derived samples and consider testing in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, from concept to clinic. We provide high-quality antibodies, including validated polyclonal formats like this anti-ARID2 antibody, to support research in chromatin biology and transcriptional regulation. Our portfolio includes bulk ancillary reagents and tailored procurement for rare targets, ensuring your diagnostic development pipeline is fully supplied.