Anti-ARID1B Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q8NFD5

Core Product Specifications and Parameters

Biological Background: ARID1B Function and Localization

• AT-rich interactive domain-containing protein 1B (ARID1B), also designated BAF250B, hOsa2, or p250R, is a subunit of the SWI/SNF ATP-dependent chromatin remodeling complex.
• Core function: ARID1B participates in transcriptional activation and repression by altering DNA–histone contacts within nucleosomes, thereby modulating chromatin accessibility.
• Neural development role: ARID1B is a component of both neural progenitors-specific (npBAF) and neuron-specific (nBAF) complexes, essential for the self-renewal of neural stem cells and later for the regulation of dendrite growth genes.
• ARID1B binds DNA non-specifically, contributing to the recruitment or stabilization of SWI/SNF complexes on chromatin. Related references: PMID:14982958, PMID:15170388
• Subcellular localization: ARID1B is predominantly nuclear, consistent with its chromatin-associated functions.
• Tissue expression: Widely expressed, with highest levels observed in heart, skeletal muscle, and kidney.
• Post-translational modifications and keywords: The protein undergoes acetylation, methylation, and phosphorylation, and is also subject to alternative initiation and splicing.
• Disease association: ARID1B mutations are linked to Coffin-Siris syndrome (CSS1) and autosomal dominant intellectual disability (MRD12), underscoring its critical role in neurodevelopment.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (amino acids 400–650), which may help minimize cross-reactivity with homologous family members. Verify sequence alignment for your species of interest.
• For Western blotting, a high molecular weight band around 244 kDa is expected. Use low-percentage polyacrylamide gels (e.g., 6–8%) and optimize transfer conditions for large proteins.
• In IHC-P and IF/ICC, nuclear staining is anticipated. Include appropriate isotype controls and validate in relevant human, mouse, or rat tissues.
• For ELISA applications, the polyclonal nature may provide robust signal. Perform bridging studies to ensure lot-to-lot consistency if used in quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with a comprehensive portfolio of IVD antibodies and raw materials. This ARID1B polyclonal antibody supports chromatin remodeling and neurobiology studies, from assay development to validation. Through our integrated supply network, we offer validated antibody pairs, monoclonal/polyclonal reagents, bulk ancillary materials, and sourcing for rare targets—always with a focus on quality and continuity for your IVD pipeline.