Anti-ARID1A Rabbit Polyclonal Antibody for WB, ELISA - O14497

Core Product Specifications and Parameters

Biological Background: ARID1A Function and Localization

• ARID1A (AT-rich interactive domain-containing protein 1A), also known as BAF250 or hOSA1, is a key subunit of SWI/SNF chromatin remodeling complexes.
• It facilitates transcriptional activation and repression by altering DNA-nucleosome contacts in an ATP-dependent manner, crucial for chromatin remodeling.
• ARID1A is a component of both neural progenitors-specific (npBAF) and neuron-specific (nBAF) chromatin remodeling complexes, essential for the self-renewal of neural stem cells and neuronal differentiation.
• Subcellular localization is primarily nuclear, consistent with its role in chromatin structure and transcription regulation.
• Tissue specificity: Highly expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood leukocytes; lower expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
• Post-translational modifications include acetylation, methylation, and phosphorylation; alternative splicing generates multiple isoforms.
• Keywords: Chromatin regulator, DNA-binding, neurogenesis, transcription, intellectual disability, among others.
• Molecular weight: Approximately 242 kDa, as determined by sequence analysis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 2195-2285 near the C-terminus; as a polyclonal antibody, it may recognize multiple epitopes, potentially enhancing signal reliability.
• For Western blot (WB), use whole-cell lysates from human, mouse, or rat tissues/cells; optimize primary antibody dilution and ensure extended transfer for the high molecular weight target (242 kDa).
• For ELISA, consider coating with recombinant ARID1A protein or peptide corresponding to the immunogen region.
• Validate expression in relevant cell lines (e.g., HeLa) or tissues where ARID1A is abundant, and include appropriate controls.
• Due to cross-reactivity with mouse and rat, confirm specificity in your experimental system if using different species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a comprehensive one-stop sourcing solution for IVD raw materials. For chromatin remodeling and neurogenesis research, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Partner with us to efficiently bridge your project from concept to clinic with reliable raw material supply.