Anti-ARHGEF9 Rabbit Polyclonal Antibody for WB, ELISA - O43307

Core Product Specifications and Parameters

Biological Background: ARHGEF9 Function and Localization

• ARHGEF9, also known as collybistin or PEM-2 homolog, is a Rho guanine nucleotide exchange factor 9 encoded by the ARHGEF9 gene (alternative names: DEE8, PEM2, EIEE8, HPEM-2, COLLYBISTIN).
• It functions as a guanine nucleotide exchange factor (GEF) for the small GTPase CDC42, facilitating the exchange of GDP for GTP to activate downstream signaling.
• ARHGEF9 promotes the formation of gephyrin (GPHN) clusters at inhibitory postsynaptic sites, playing a key role in synaptic organization.
• Subcellularly, it localizes to the cytoplasm and is enriched at the postsynaptic density.
• Tissue expression is predominantly in the brain, with low-level detection in the heart.
• Post-translational modifications include phosphorylation, and the protein is subject to alternative splicing, yielding multiple isoforms.
• Clinically, mutations in ARHGEF9 are associated with epileptic encephalopathy, early infantile, 8 (EIEE8) and other neurodevelopmental disorders, highlighting its critical role in neuronal function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 307–516, located within the C-terminal region; epitope accessibility may differ between native and denatured protein contexts in assays like WB or ELISA.
• For Western blotting, a band at approximately 61 kDa is expected; consider using reducing conditions and validating with brain tissue lysates where ARHGEF9 is highly expressed.
• In ELISA, direct coating with recombinant protein or peptide may help optimize signal; titration of both antibody and antigen is recommended.
• Reported cross-reactivity with mouse and rat enables multispecies studies, but researchers should confirm reactivity in their specific experimental model due to potential species-specific expression differences.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. This ARHGEF9 antibody, targeting a key synaptic protein in neuroscience, exemplifies our capability to deliver validated polyclonal antibodies for critical research areas. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing of rare target raw materials to streamline your assay development and production needs.