Anti-ARHGEF9 Rabbit Polyclonal Antibody for IF/ICC and ELISA - O43307

Core Product Specifications and Parameters

Biological Background: ARHGEF9 Function and Localization

• ARHGEF9 (also known as Collybistin, PEM-2, or KIAA0424) is a Rho guanine nucleotide exchange factor belonging to the Dbl family.
• It acts as a guanine nucleotide exchange factor (GEF) for the small GTPase CDC42, facilitating the exchange of GDP for GTP.
• ARHGEF9 promotes the formation of gephyrin (GPHN) clusters, which are critical for inhibitory synapse formation and function.
• The protein contains an SH3 domain and is subject to alternative splicing, producing multiple isoforms.
• Subcellularly, ARHGEF9 localizes to the cytoplasm and the postsynaptic density of inhibitory synapses.
• Tissue expression is predominantly in the brain, with low levels detected in the heart.
• Post-translational modifications include phosphorylation, and mutations in ARHGEF9 are associated with epileptic encephalopathy.
• Keywords: 3D-structure, Alternative splicing, Cytoplasm, Disease variant, Epilepsy, Guanine-nucleotide releasing factor, Phosphoprotein, Proteomics identification, Reference proteome, SH3 domain, Synapse.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fusion protein covering amino acids 7-75 of human ARHGEF9, targeting the N-terminal region.
• For immunofluorescence/immunocytochemistry (IF/ICC), consider permeabilization and validate in neuronal cell lines or brain tissue sections to detect synaptic localization.
• For ELISA, optimize the antibody concentration and assay conditions for your specific sample type and plate format.
• As a polyclonal antibody, it may recognize multiple epitopes; verify specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, recombinant proteins, and ancillary reagents to support your assay development. For synaptic research targets like ARHGEF9, we provide optimized antibodies and bulk quantities tailored to your needs. From concept to clinic, CamelBio bridges your research to reliable, scalable supply chains.