Anti-ARHGEF7 Rabbit Polyclonal Antibody for WB, ELISA - Q14155

Core Product Specifications and Parameters

Biological Background: ARHGEF7 Function and Localization

• ARHGEF7, also known as Beta-Pix or COOL-1, is a Rho guanine nucleotide exchange factor (GEF) that specifically activates RAC1, a small GTPase involved in cytoskeletal dynamics.
• It plays a critical role in cell migration, attachment, and spreading by promoting RAC1 targeting to focal adhesions and inducing membrane ruffling.
• ARHGEF7 functions downstream of NMDA receptors and the CaMKK-CaMK1 signaling cascade in hippocampal neurons, where it promotes the formation of dendritic spines and synapses.
• It may act as a positive regulator of apoptosis, though the mechanisms remain to be fully elucidated.
• The protein localizes to cell junctions, focal adhesions, ruffles, the cell cortex, and lamellipodia, consistent with its role in cell adhesion and motility.
• ARHGEF7 contains an SH3 domain and undergoes post-translational modifications including acetylation and phosphorylation, with multiple isoforms produced by alternative splicing.
• Its molecular weight is approximately 90 kDa, and it is classified under keywords such as guanine-nucleotide releasing factor and neurogenesis.

Experimental Guidance and Technical Tips

• For Western blotting, a band around 90 kDa is expected; consider using appropriate molecular weight markers and validating in human and mouse cell lysates.
• The immunogen covers the N-terminal region (aa 1-260), which may target specific isoforms; this could influence detection depending on the isoform expression in your samples.
• For ELISA, consider using the antibody in combination with a detection system optimized for rabbit polyclonal antibodies, and validate specificity with recombinant protein or peptide blocking.
• As a polyclonal antibody, batch-to-batch consistency should be evaluated for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to provide a comprehensive range of IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this ARHGEF7 antibody. We support the sourcing of rare-target reagents for cell migration and signaling studies, alongside bulk ancillary reagents to streamline your assay development and supply chain.