Anti-ARHGEF3 Rabbit Polyclonal Antibody for WB and ELISA - Q9NR81

Core Product Specifications and Parameters

Biological Background: ARHGEF3 Function and Localization

• ARHGEF3 (Rho guanine nucleotide exchange factor 3) is also known as Exchange factor found in platelets and leukemic and neuronal tissues (XPLN), and is a member of the Dbl family of GEFs.
• It functions as a guanine nucleotide exchange factor (GEF) specifically activating the small GTPases RhoA and RhoB, thereby promoting their transition from an inactive GDP-bound state to an active GTP-bound state.
• By activating RhoA and RhoB, ARHGEF3 regulates actin cytoskeleton dynamics, cell morphology, migration, and various signaling pathways.
• The protein is widely expressed across human tissues, with highest levels observed in adult brain and skeletal muscle, and lower levels in heart and kidney.
• ARHGEF3 localizes predominantly to the cytoplasm.
• Post-translational modifications include phosphorylation, and the gene undergoes alternative splicing.
• It is identified in proteomic studies and is a reference proteome member.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 287-526 of ARHGEF3, which encompasses a region likely critical for GEF activity; this may provide specific detection of the target.
• For Western blot, consider using whole cell lysates from human or mouse tissues/cell lines expressing ARHGEF3 (e.g., brain, skeletal muscle) and validate band size around 60 kDa.
• This polyclonal antibody may also be suitable for ELISA; optimize coating and detection conditions based on your assay requirements.
• Cross-reactivity with mouse has been confirmed, so consider appropriate sample species during experimental design.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This ARHGEF3 polyclonal antibody supports research into Rho GTPase signaling and related pathways.