Anti-ARHGEF2 Rabbit Polyclonal Antibody for WB and ELISA - Q92974

Core Product Specifications and Parameters

Biological Background: ARHGEF2 Function and Localization

• ARHGEF2, also known as GEF-H1, Lfc, or p40, is a Rho guanine nucleotide exchange factor that catalyzes GDP/GTP exchange on Rho-GTPases, playing pivotal roles in cytoskeletal dynamics, cell motility, and polarization.
• It regulates epithelial barrier function by modulating tight junctions and paracellular permeability; overexpression increases permeability, and its activity is controlled by microtubule binding.
• ARHGEF2 serves as a critical innate immune sensor: it collaborates with NOD1 to detect microbial effectors during pathogen invasion (e.g., Shigella flexneri) and mediates NF-kappaB activation via RHOA and RIPK2.
• In macrophages, ARHGEF2 transduces signals from NOD2 to RIPK2 upon peptidoglycan stimulation, leading to phosphorylation of RIPK2 by Src kinase and consequent NF-kappaB activation, which drives IL-6 and TNF secretion.
• While an early study suggested nucleotide exchange activity toward Rac-GTPases (PubMed:9857026), ARHGEF2 primarily activates Rho-GTPases and may instead stimulate cortical Rac activity. Related references: PMID:9857026
• ARHGEF2 contributes to neuronal development, including progenitor cell division and differentiation (PubMed:28453519) and the migration of precerebellar neurons (By similarity). Related references: PMID:28453519
• Subcellularly, ARHGEF2 localizes to the cytoplasm, cytoskeleton (microtubules, spindle), tight junctions, Golgi apparatus, ruffle membrane, and cytoplasmic vesicles, consistent with its dynamic regulatory roles.
• The protein undergoes post-translational modifications such as acetylation and phosphorylation, and contains characteristic domains including a DH/PH tandem and a zinc finger motif.

Experimental Guidance and Technical Tips

• For Western blot, use reducing conditions and a positive human cell lysate (e.g., HeLa, HEK293) to detect endogenous ARHGEF2 at the expected molecular weight of 112 kDa; validate in your specific model system.
• As a polyclonal antibody, it may recognize multiple epitopes enhancing sensitivity, but lot-to-lot variation should be monitored by parallel testing of a reference standard.
• In ELISA, this antibody can be employed as a capture or detection reagent; titrate the antibody and optimize blocking/detection conditions for your assay format.
• The immunogen was raised against a C-terminal peptide (aa 850–950), suggesting reactivity with both full-length and potential C-terminal splice variants; confirm specificity via knockout or siRNA controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, providing diagnostic developers with validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target sourcing services. This ARHGEF2 antibody supports research in cell signaling, immunology, and oncology, and our team can assist in custom reagent procurement to accelerate your assay development from concept to clinic.