Anti-ARHGEF11 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O15085

Core Product Specifications and Parameters

Biological Background: ARHGEF11 Function and Localization

• ARHGEF11, also known as PDZ-RhoGEF or GTRAP48, is a Rho guanine nucleotide exchange factor encoded by the ARHGEF11 gene (KIAA0380) in humans.
• It functions as a guanine nucleotide exchange factor (GEF) that specifically activates RhoA GTPase by promoting the exchange of GDP for GTP.
• Additionally, ARHGEF11 may act as a GTPase-activating protein (GAP) for the heterotrimeric G proteins GNA12 and GNA13, linking GPCR signaling to RhoA activation.
• The protein is involved in neurotrophin-induced neurite outgrowth, suggesting a role in neuronal development and axon guidance.
• ARHGEF11 localizes to the cytoplasm and membrane, consistent with its function in signal transduction at the plasma membrane.
• It is ubiquitously expressed across human tissues, indicating broad regulatory roles.
• Post-translational modifications include phosphorylation (phosphoprotein) and ubiquitination (ubl conjugation), which may regulate its activity or stability.
• Alternative splicing produces multiple isoforms, and the protein features coiled-coil domains and a PDZ domain, important for protein interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide within the N-terminal region (aa 1-100) of human ARHGEF11; following primary immunization and purification, the resulting polyclonal antibody contains a mixture of immunoglobulins recognizing multiple epitopes within this region.
• For Western blot (WB), consider using a reduced denaturing gel and verify the band at the predicted molecular weight of approximately 168 kDa. Validate specificity using knockdown or knockout controls if available.
• For immunohistochemistry (IHC-P), antigen retrieval protocols (e.g., heat-induced epitope retrieval in citrate buffer pH 6.0) and optimization of primary antibody dilution are recommended. Include appropriate positive and negative tissue controls.
• For ELISA, the antibody can be used as capture or detection reagent; however, assay conditions such as coating buffer, blocking, and detection system must be optimized for each specific setup.
• Given the cross-reactivity with mouse, this antibody may be useful for comparative studies, but users should confirm reactivity in their species and application of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with reliable access to high-quality IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. For studies on Rho GTPase signaling and neuronal development, we offer a range of antibodies targeting ARHGEF11 and related pathways, along with customized sourcing for rare or custom targets. Contact us to streamline your raw-material procurement from discovery to clinical application.