Anti-ARHGEF10L Polyclonal Antibody (Rabbit) for WB, ELISA - Q9HCE6

Core Product Specifications and Parameters

Biological Background: ARHGEF10L Function and Localization

• ARHGEF10L, also known as GrinchGEF, is a Rho guanine nucleotide exchange factor (GEF) that specifically activates RHOA, RHOB, and RHOC by catalyzing the exchange of GDP for GTP.
• It belongs to the Dbl family of guanine nucleotide exchange factors and plays a role in cytoskeletal reorganization and cell signaling pathways mediated by Rho GTPases.
• The protein is encoded by the ARHGEF10L gene, which undergoes alternative splicing to generate multiple transcript variants.
• Subcellularly, ARHGEF10L localizes predominantly to the cytoplasm, consistent with its role as a cytoplasmic signaling protein.
• Post-translational modifications include phosphorylation; phosphoproteomic studies have identified this protein as a phosphoprotein.
• Tissue expression analysis reveals that ARHGEF10L is detected in heart, liver, skeletal muscle, kidney, and pancreas.
• Keywords associated with this protein include Alternative splicing, Cytoplasm, Guanine-nucleotide releasing factor, Phosphoprotein, and Reference proteome, reflecting its biological characteristics and curated status.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fusion protein covering amino acids 1120-1230 of human ARHGEF10L. This region may serve as the primary epitope for antibody recognition.
• In Western blot, ARHGEF10L has a predicted molecular weight of approximately 140 kDa; consider optimizing gel percentage and transfer conditions for high molecular weight proteins.
• For ELISA, it is advisable to validate the antibody using the immunogen or cell lysates expressing the target protein.
• The antibody is reported to cross-react with human and rat species; cross-reactivity with other species should be experimentally verified.
• As a polyclonal antibody, it may recognize multiple epitopes; include appropriate negative and positive controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers with high-quality reagents. For targets like ARHGEF10L, which are involved in Rho GTPase signaling and cytoskeletal regulation, CamelBio can supply validated antibody pairs, batch-optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Leveraging its one-stop platform, CamelBio also assists in sourcing rare target raw materials, helping to streamline your development from concept to clinic.