Anti-ARHGAP44 Polyclonal Antibody for WB and ELISA - Q17R89

Core Product Specifications and Parameters

Biological Background: ARHGAP44/RICH2 Function and Localization

• ARHGAP44/RICH2 is a Rho GTPase-activating protein that stimulates the GTPase activity of CDC42 and RAC1, thereby inactivating these small GTPases (PubMed:11431473). Related references: PMID:11431473
• The protein plays a critical role in neuronal dendritic spine formation and synaptic plasticity by regulating RAC1-dependent actin dynamics (By similarity).
• ARHGAP44 limits the initiation of exploratory dendritic filopodia by localizing to actin patches and reducing actin polymerization through its GAP activity on RAC1 (By similarity).
• In association with SHANK3, ARHGAP44 promotes GRIA1 exocytosis from recycling endosomes and contributes to spine morphological changes during long-term potentiation (By similarity).
• Subcellularly, ARHGAP44 is found at dendritic spines, the presynapse, recycling endosomes, and dendritic compartments.
• Expression is highly enriched in the brain, with only weak levels detected in non-neural tissues.
• The protein is subject to alternative splicing and phosphorylation, as identified by proteomics, suggesting regulatory post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 666-772 of human ARHGAP44. This region may not span known functional domains; therefore, consider mapping the epitope if precise domain-specific detection is required.
• For Western blot (WB), ARHGAP44 has a predicted molecular weight of approximately 89 kDa. Validate band specificity using appropriate positive controls (e.g., brain lysates) and negative controls (non-neural tissues) given its brain-enriched expression.
• In ELISA applications, the antibody can be used for antigen capture or detection; however, optimal dilution and buffer conditions should be empirically determined.
• Cross-reactivity with mouse and rat is indicated, making this antibody suitable for comparative studies in rodent models. Confirm reactivity with your specific sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As your one-stop sourcing partner, CamelBio offers a comprehensive portfolio of IVD raw materials, including this ARHGAP44 polyclonal antibody, to support neuroscience and cell signaling research. Our supply capabilities encompass validated antibody pairs, bulk reagents, and customized sourcing for rare targets like Rho GTPase regulators, helping streamline your assay development from concept to clinic.