Anti-ARHGAP4 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P98171

Core Product Specifications and Parameters

Biological Background: ARHGAP4 Function and Localization

• ARHGAP4, also known as Rho GTPase-activating protein 4, p115, RGC1, or SrGAP4, is encoded by the ARHGAP4 gene (KIAA0131, RGC1, RHOGAP4) in humans.
• It functions as a GTPase-activating protein (GAP) for Rho family GTPases, exerting an inhibitory effect on stress fiber organization and potentially down-regulating Rho-like GTPase activity in hematopoietic cells.
• ARHGAP4 contains a coiled-coil domain and an SH3 domain, which may mediate protein-protein interactions, and is subject to alternative splicing and phosphorylation.
• The protein is predominantly expressed in hematopoietic cells, including spleen, thymus, and leukocytes, with lower levels in placenta, lung, and fetal tissues, indicating a specialized role in blood cell biology.
• Subcellularly, ARHGAP4 localizes to the cytoplasm, where it likely interacts with Rho GTPases and cytoskeletal components to regulate cell morphology and motility.
• Structural studies (3D-structure available) have provided insights into its domain organization and catalytic mechanism, aiding in understanding its role in signal transduction.
• As a reference proteome member, ARHGAP4 has been identified in proteomics studies, underscoring its expression and potential functional relevance in various cellular contexts.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 900–986) of human ARHGAP4 (isoform 1, NP_001158213.1), which is unique to this isoform and may allow isoform-specific detection.
• For Western blot (WB), the predicted molecular weight is ~105 kDa; ensure appropriate gel percentage and membrane transfer conditions for high-molecular-weight proteins.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate antibody specificity using appropriate controls (e.g., blocking peptide or knockout tissue/cells) due to potential cross-reactivity with other RhoGAP family members.
• For ELISA, this polyclonal antibody may serve as a capture or detection reagent; optimization of coating and detection conditions is recommended.
• Since ARHGAP4 is predominantly expressed in hematopoietic cells, consider testing positive controls from spleen, thymus, or leukocyte lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a comprehensive suite of IVD raw materials and support services. For ARHGAP4-related studies in hematopoietic cell biology and Rho GTPase signaling, we offer validated antibody pairs, bulk ancillary reagents, and expert assistance in sourcing rare-target raw materials. Partner with CamelBio to streamline your assay development from concept to clinic.