Anti-ARHGAP25 Rabbit Polyclonal Antibody for WB, IHC-P - P42331

Core Product Specifications and Parameters

Biological Background: ARHGAP25 Function and Localization

• ARHGAP25 (also known as KIAA0053) is a member of the Rho GTPase-activating protein family that specifically stimulates the intrinsic GTP hydrolysis of Rho-type GTPases, converting them to an inactive GDP-bound state and thereby regulating actin cytoskeleton dynamics and cell signaling.
• The canonical protein predicted from UniProt accession P42331 has a molecular weight of approximately 73.4 kDa, consistent with the observed product weight of 73 kDa.
• The protein contains a coiled-coil domain, which likely mediates protein-protein interactions essential for its cellular functions.
• ARHGAP25 is annotated as a phosphoprotein, indicating that its activity or localization may be modulated by phosphorylation.
• Alternative splicing of the ARHGAP25 gene produces multiple isoforms, potentially with distinct regulatory properties.
• A three-dimensional structure of ARHGAP25 is available, offering molecular insights into its catalytic mechanism and structural features.
• The protein is classified under the reference proteome, ensuring high-confidence annotation of its sequence and functional domains.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (amino acids 1-370) of human ARHGAP25 isoform 1, encompassing the coiled-coil domain and likely recognizing all major isoforms if the epitopes are preserved.
• For western blot analysis, a major band at around 73 kDa is expected; additional bands may appear due to alternative splicing or post-translational modifications.
• When performing IHC-P or IF/ICC, include appropriate positive and negative controls from tissues or cell lines known to express ARHGAP25, and consider optimizing antigen retrieval and permeabilization conditions.
• Validate antibody specificity in the relevant species (human, mouse) using knockdown or knockout controls if available, and determine optimal antibody dilutions experimentally for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For projects involving Rho GTPase signaling and cytoskeletal regulation, we can source rare-target antibodies like this ARHGAP25 polyclonal antibody efficiently. Our supply chain expertise ensures reliable procurement to accelerate your assay development from concept to clinic.