Anti-ARHGAP25 Rabbit Polyclonal Antibody for WB, ELISA - P42331

Core Product Specifications and Parameters

Biological Background: ARHGAP25 Function and Localization

• ARHGAP25 encodes a Rho GTPase-activating protein that negatively regulates Rho family GTPases by stimulating GTP hydrolysis to maintain an inactive GDP-bound state.
• Alternative splicing of ARHGAP25 generates multiple transcript variants, potentially encoding distinct protein isoforms with tissue-specific functions.
• The protein contains a coiled coil domain, which may facilitate protein-protein interactions and subcellular targeting.
• ARHGAP25 has been identified through proteomics identification studies, indicating its presence across diverse human tissue proteomes.
• As a phosphoprotein, ARHGAP25 may undergo phosphorylation-dependent regulation, influencing its GTPase-activating activity or localization.
• Partial 3D-structure determination of ARHGAP25 provides insights into its domain architecture and catalytic mechanism.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–370, covering the N-terminal region of ARHGAP25; consider this when interpreting results in samples with potential N-terminal processing or truncation.
• For Western blot, ARHGAP25 is predicted to migrate at approximately 73 kDa; validate antibody specificity using positive and negative control lysates from human, mouse, or rat cell lines.
• When performing ELISA, optimize coating antigen and detection conditions to achieve linear detection of recombinant or endogenous ARHGAP25.
• Verify cross-reactivity in your specific experimental system; although the antibody is reported to detect human, mouse, and rat ARHGAP25, performance may vary by sample type and preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams streamlined access to validated IVD raw materials like this ARHGAP25 antibody for GTPase signaling research. Our offerings include optimized polyclonal and monoclonal antibodies, matched antibody pairs, and bulk ancillary reagents—supported by sourcing capabilities for rare targets. From concept to clinic, we serve as a reliable bridge to accelerate your assay development.