Anti-ARHGAP22 Polyclonal Antibody for WB, ELISA - Q7Z5H3

Core Product Specifications and Parameters

Biological Background: ARHGAP22 Function and Localization

• ARHGAP22, also known as RhoGAP2 or RhoGap22, is a Rho GTPase-activating protein involved in angiogenesis and transcription regulation.
• It functions as a GTPase activator for RAC1, converting it to an inactive GDP-bound state, thereby inhibiting RAC1-dependent lamellipodia formation.
• ARHGAP22 plays a role in endothelial cell capillary tube formation during angiogenesis.
• The protein may also regulate transcription by interacting with VEZF1 and modulating the activity of the endothelin-1 (EDN1) promoter.
• ARHGAP22 localizes to both the cytoplasm and nucleus.
• Post-translational modifications include phosphorylation, and the protein undergoes alternative splicing.
• It contains a coiled-coil domain and is associated with developmental processes and differentiation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fragment covering amino acids 510-650 of human ARHGAP22 (isoform NP_001242953.1). Consider verifying specificity in samples expressing this region, particularly if alternative splicing isoforms are present.
• For Western blotting, human, mouse, and rat cell lysates can be used as positive controls. The predicted molecular weight is ~77 kDa, but actual migration may differ due to phosphorylation or other modifications.
• In ELISA, validate the antibody with the immunogen or full-length recombinant protein to ensure optimal signal-to-noise ratio.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical applications; consider requesting retained samples from the same lot for longitudinal studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and labs with a one-stop supply of IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This anti-ARHGAP22 polyclonal antibody supports angiogenesis and transcription regulation research. Our sourcing platform can also accommodate rare-target and custom antibody requirements, streamlining your R&D and IVD development pipeline.