Anti-ARHGAP11B Rabbit Polyclonal Antibody for WB, ELISA - Q3KRB8

Core Product Specifications and Parameters

Biological Background: ARHGAP11B Function and Localization

• ARHGAP11B is an inactive Rho GTPase-activating protein 11B, also referred to as Rho-type GTPase-activating protein 11B.
• This hominin-specific protein is essential for evolutionary expansion of the brain neocortex, promoting basal progenitor amplification and increasing neuron production during fetal corticogenesis. Related references: PMID:25721503 PMID:27957544 PMID:30484771
• It promotes basal progenitor proliferation by inhibiting the mitochondrial permeability transition pore (mPTP) through interaction with ADP:ATP translocase, thereby raising mitochondrial Ca²⁺ levels and driving glutamine catabolism. Related references: PMID:31883789
• The lack of GTPase activator activity is not a defect but a prerequisite for its role in basal progenitor amplification. Related references: PMID:25721503 PMID:27957544
• ARHGAP11B localizes to the mitochondrial matrix, consistent with its involvement in mPTP regulation.
• Annotated keywords include GTPase activation, Mitochondrion, Reference proteome, and Transit peptide.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 218–267, a region unique to human ARHGAP11B; ensure sequence alignment if using non-human samples.
• For WB, consider using whole cell lysates from human brain tissue or neuron-derived cell lines, where target expression is expected.
• Given the hominin specificity, cross-reactivity with rodent or other model organisms is unlikely; always include species-appropriate controls to verify reactivity.
• As a polyclonal antibody, batch-to-batch consistency should be validated for critical quantitative applications.
• Optimize antibody dilution and incubation conditions in your specific experimental system; recommended starting dilutions are not provided and must be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This ARHGAP11B antibody supports neuroscientific and developmental biology research applications, backed by our comprehensive supply capabilities. Contact us for tailored procurement solutions and technical support to accelerate your diagnostic development.