Anti-Argonaute-2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9UKV8

Core Product Specifications and Parameters

Biological Background: Argonaute-2 Function and Localization

• Protein argonaute-2 (AGO2), also known as Argonaute RISC catalytic component 2, eIF2C2, PPD, and Protein slicer, is the central catalytic component of the RNA-induced silencing complex (RISC).
• AGO2 is essential for RNA-mediated gene silencing (RNAi). It binds short guide RNAs such as miRNA or siRNA, which direct RISC to complementary target mRNAs.
• Upon binding to a perfectly complementary target mRNA, AGO2 mediates endonucleolytic cleavage (slicer activity), leading to rapid mRNA degradation.
• For partially complementary targets, AGO2 inhibits translation initiation by binding the 7-methylguanosine cap or through interaction with EIF6, independent of its endonuclease activity.
• AGO2 localizes to the cytoplasm, particularly in processing bodies (P-bodies), and also shuttles to the nucleus, where it participates in transcriptional gene silencing.
• Post-translational modifications including hydroxylation, phosphorylation, nitration, and ubiquitination regulate AGO2 stability, activity, and interactions.
• Alternative splicing generates multiple AGO2 isoforms, potentially diversifying its functional roles.
• Upon SARS-CoV-2 infection, AGO2 associates with a viral miRNA-like small RNA (CoV2-miR-O7a) and may repress host antiviral genes, aiding immune evasion.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide matching residues 1-100 of human AGO2; thus, the antibody likely recognizes the N-terminal region. This may be useful for detecting both full-length and N-terminal fragments.
• For Western blot (WB), AGO2 migrates at approximately 97 kDa; consider using an appropriate molecular weight marker and optimizing protein loading and blocking conditions.
• In immunohistochemistry (IHC-P), validate the antibody in your tissue of interest using appropriate antigen retrieval and detection methods; endogenous peroxidase blocking may be required.
• For ELISA, the polyclonal nature may provide robust signal across sample types; consider performing a checkerboard titration to determine optimal antibody and antigen concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive IVD raw-material procurement, including validated antibody pairs and optimized polyclonal/monoclonal antibodies like this anti-Argonaute-2 pAb. Extending beyond standard catalog items, CamelBio facilitates sourcing of rare target materials, bulk ancillary reagents, and tailored solutions, streamlining your workflow from early-stage research to clinical assay development in the RNAi and gene silencing fields.