Anti-Argonaute-2 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9UKV8

Core Product Specifications and Parameters

Biological Background: Argonaute-2 Function and Localization

• Protein Argonaute-2 (AGO2, also known as eIF2C2) is the catalytic component of the RNA-induced silencing complex (RISC) essential for RNA interference (RNAi).
• AGO2 binds short guide RNAs (miRNAs or siRNAs) to direct RISC to complementary mRNAs, leading to post-transcriptional gene silencing through endonucleolytic cleavage (slicing) or translational repression, depending on complementarity.
• It mediates translational inhibition by binding the mRNA cap, preventing eIF4E recruitment, and via interaction with EIF6, which blocks ribosomal subunit joining; targeted mRNAs accumulate in cytoplasmic P-bodies.
• AGO2 can also up-regulate translation of specific mRNAs (e.g., TNF) under serum starvation, and participates in transcriptional gene silencing (TGS) by targeting promoter regions with antigene RNAs.
• During SARS-CoV-2 infection, AGO2 associates with viral miRNA-like small RNA CoV2-miR-O7a to repress host mRNAs such as BATF2, contributing to evasion of interferon response.
• Subcellularly, AGO2 localizes to the cytoplasm (including P-bodies) and the nucleus.
• Post-translational modifications include phosphorylation, hydroxylation, nitration, and ubiquitination; it requires magnesium or manganese for endonuclease activity.
• Keywords highlight its roles in RNA-binding, ribonucleoprotein complex, host-virus interaction, and disease association (intellectual disability).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from a conserved region (amino acids 101-200), consistent with detection of human, mouse, and rat AGO2; however, always validate specificity in the relevant sample system.
• For Western blot (WB), the expected band is ~97 kDa; include appropriate positive and negative controls and consider using a loading control for normalization.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and antibody dilutions using known AGO2-expressing tissues or cells.
• ELISA applications may require further optimization; consider checkerboard titrations to determine optimal coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing platform for IVD raw materials, covering from concept to clinic. This Argonaute-2 antibody supports research in RNA interference, gene silencing, and viral-host interactions, with reliable bulk supply and technical consultation. Our offerings include validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents to accelerate your diagnostic development.