Anti-Argonaute-2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9UKV8

Core Product Specifications and Parameters

Biological Background: Argonaute-2 Function and Localization

• Argonaute-2 (Ago2) is the catalytic component of the RNA-induced silencing complex (RISC), essential for RNA interference (RNAi) and microRNA (miRNA)-mediated gene silencing.
• It binds short guide RNAs (siRNAs and miRNAs) and directs RISC to complementary mRNA targets, leading to endonucleolytic cleavage when base-pairing is perfect, or translational repression and mRNA decay when pairing is imperfect.
• Belonging to the Argonaute protein family, AGO2 (also known as EIF2C2) is the only human Argonaute with demonstrated slicer activity.
• Multiple protein isoforms are produced through alternative splicing, and several aliases exist (e.g., PPD, Q10, CASC7).
• Subcellular localization includes the cytoplasm, P-bodies (cytoplasmic processing bodies), and the nucleus, reflecting its dynamic roles in post-transcriptional and transcriptional regulation.
• AGO2 also participates in transcriptional gene silencing (TGS) by targeting promoter regions with antigene RNAs.
• During SARS‑CoV‑2 infection, AGO2 associates with a viral miRNA-like small RNA (CoV2‑miR‑O7a) to repress host mRNAs and evade the interferon response.
• Post-translational modifications include phosphorylation, hydroxylation, nitration, and ubiquitination, which may regulate its stability, localization, and activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 350–450 of human Argonaute-2, a region that may be conserved across splice variants; however, isoform specificity should be verified in your experimental system.
• For Western blot, a band around 97 kDa is expected. Use reducing conditions and include positive control lysates from human, mouse, or rat cells.
• In immunohistochemistry (paraffin-embedded sections), optimize antigen retrieval and antibody dilution; cross-reactivity with mouse and rat supports comparative tissue studies.
• When developing an ELISA, pairing this monoclonal with a suitably matched detection antibody is recommended; validate against known standards in your specific assay format.
• Because Argonaute-2 participates in dynamic RISC complexes, consider native immunoprecipitation conditions if you plan to study protein‑protein or protein‑RNA interactions.

CamelBio: Your One-Stop Sourcing Bridge

This anti-Argonaute-2 monoclonal antibody aids research and diagnostic applications in RNA interference, gene regulation, and host‑virus interactions. As your dedicated IVD raw‑material partner, CamelBio supplies validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing solutions. From early-stage assay development to clinical readiness, we provide reliable access to critical reagents, including rare targets like Argonaute-2.