Anti-Argonaute-2 (EIF2C2) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UKV8

Core Product Specifications and Parameters

Biological Background: Argonaute-2 Function and Localization

• Argonaute-2 (AGO2, EIF2C2) is the catalytic component of the RNA-induced silencing complex (RISC) responsible for RNA interference (RNAi).
• It binds small guide RNAs (miRNAs, siRNAs) to mediate post-transcriptional gene silencing via endonucleolytic cleavage or translational repression, depending on target complementarity.
• AGO2 shuttles between the cytoplasm, P-bodies, and nucleus, enabling both cytoplasmic mRNA silencing and transcriptional gene silencing (TGS) in the nucleus.
• Post-translational modifications, including phosphorylation, hydroxylation, nitration, and ubiquitination, modulate AGO2 stability and function.
• During SARS-CoV-2 infection, AGO2 interacts with viral miRNA CoV2-miR-O7a to suppress host IFN response by targeting BATF2 mRNA.
• AGO2 belongs to the Argonaute protein family, featuring PAZ and Piwi domains; the Piwi domain provides the endonuclease active site dependent on divalent metal ions (Mg²⁺/Mn²⁺).
• Mutations in the AGO2 gene are linked to neurodevelopmental disorders, including intellectual disability.

Experimental Guidance and Technical Tips

• For Western blot, start with a 1:500–1:1000 dilution and validate in your sample type; expect a band around 97 kDa.
• For immunofluorescence, use suitable permeabilization to detect AGO2 in the cytoplasm, P-bodies, or nucleus depending on cell context.
• Confirm cross-reactivity with mouse and rat tissues by testing in the relevant species, as sequence homology supports binding.
• Because the immunogen covers the N-terminal region (aa 47–115), the antibody may recognize multiple isoforms; verify band pattern in your system.
• For ELISA, optimize coating conditions and consider pairing with a matched detection antibody for sandwich applications.
• Perform appropriate controls for phospho-specificity if studying modification-dependent localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with end-to-end IVD raw material solutions. This Argonaute-2 polyclonal antibody is part of a broad portfolio that includes validated antibody pairs, recombinant monoclonal antibodies, and bulk ancillary reagents for RNAi and gene silencing studies. Our one-stop sourcing services streamline procurement of rare-target raw materials, technical consulting, and assay development support from concept to clinic.