Anti-Arginase 2 (ARG2) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P78540

Core Product Specifications and Parameters

Biological Background: Arginase 2 (ARG2) Function and Localization

• Arginase-2 (ARG2) is a mitochondrial manganese-dependent hydrolase that converts L-arginine to urea and L-ornithine, playing a central role in extra-urea cycle arginine metabolism and regulation of nitric oxide synthesis.
• Subcellular localization: ARG2 localizes primarily to the mitochondrion.
• Tissue specificity: ARG2 is most highly expressed in kidney and prostate, with lower expression in brain, skeletal muscle, placenta, lung, macrophages, and activated T cells.
  Related references: PMID:27745970
• ARG2 negatively regulates the survival of activated CD4+ and CD8+ T cells, thereby modulating adaptive immune responses.
  Related references: PMID:27745970
• The enzyme suppresses inflammation-related signaling in the asthmatic airway epithelium.
  Related references: PMID:27214549
• In fetal dendritic cells, ARG2 promotes immune suppression and enhances T cell TNF production during gestation.
  Related references: PMID:28614294
• ARG2 promotes endothelial cell senescence and inflammation via RPS6KB1 signaling (PubMed:22928666), inhibits endothelial autophagy independently of its enzymatic activity through mTORC2 (PubMed:25484082), and mediates vascular smooth muscle cell senescence and apoptosis (PubMed:23832324).
  Related references: PMID:22928666 | PMID:25484082 | PMID:23832324
• ARG2 plays a role in both male and female sexual arousal by modulating NOS activity in penile and clitoral cavernosum smooth muscle.
  Related references: PMID:12859189

Experimental Guidance and Technical Tips

• The immunogen peptide corresponds to amino acids 255-354, a region within the C-terminal portion of ARG2. This may offer good antigenicity and accessibility.
• Predicted molecular weight is approximately 39 kDa; a reducing SDS-PAGE gel with appropriate molecular weight markers is recommended for Western blot analysis.
• For Western blotting (WB), consider using lysates from kidney or prostate tissues as positive controls, given the high expression of ARG2 in these tissues. Validate antibody specificity with a negative control lacking ARG2 expression.
• For immunohistochemistry (IHC-P), standard antigen retrieval methods (e.g., heat-induced epitope retrieval) may be required. Optimize staining conditions using ARG2-positive tissue sections.
• For ELISA, optimize coating antigen concentration and antibody titration to achieve optimal signal-to-noise ratio.
• The antibody is specific for human ARG2; cross-reactivity with other species has not been tested and should be validated independently if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibodies like this anti-ARG2 monoclonal antibody. Whether you require antibody pairs, bulk ancillary reagents, or rare-target sourcing for arginine metabolism and immune regulation studies, CamelBio provides tailored solutions to streamline your assay development from concept to clinic.