Anti-Arginase-1 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q61176

Core Product Specifications and Parameters

Biological Background: Arginase-1 Function and Localization

• Arginase-1 is a manganese-dependent hydrolase that catalyzes the conversion of L-arginine to urea and L-ornithine, a key reaction in the hepatic urea cycle for ammonia detoxification.
• Beyond the liver, it regulates L-arginine homeostasis in non-hepatic tissues, competing with nitric oxide synthase (NOS) for the shared substrate arginine, and thereby modulating nitric oxide production.
• In immune cells, notably alternatively activated M2 macrophages, arginase-1 supports wound healing, tissue regeneration, and immune suppression; its function is organ-specific and influences anti-parasitic and allergic responses. Related references: PMID:19360123, PMID:20483789, PMID:23552798
• Within the tumor microenvironment, it is expressed by infiltrating dendritic cells and myeloid-derived suppressor cells, where it dampens T cell-mediated antitumor immunity through arginine depletion. Related references: PMID:19414774, PMID:23248265
• In group 2 innate lymphoid cells (ILC2s), arginase-1 promotes acute type 2 lung inflammation and fuels optimal ILC2 proliferation, linking arginine metabolism to innate lymphoid cell function. Related references: PMID:27043409
• Subcellularly, it localizes to the cytoplasm and cytoplasmic granules, consistent with its role in rapid arginine metabolism upon release.
• Tissue expression is prominent in macrophages, ILC2s, and lung tumor-associated myeloid cells, reflecting its immunological and metabolic significance. Related references: PMID:12193690, PMID:19360123, PMID:7537672, PMID:27043409, PMID:15313928, PMID:19414774

Experimental Guidance and Technical Tips

• The immunogen is a recombinant mouse Arginase-1 protein; verify cross-reactivity with rat samples by sequence alignment, and consider using positive controls from mouse liver or macrophage lysates.
• For western blot (WB), expect a band at approximately 34 kDa; include negative controls (e.g., Arginase-1 knockout cell lines) to confirm specificity.
• In immunofluorescence (IF-P), optimize fixation (e.g., 4% PFA) and permeabilization due to the cytoplasmic localization; counterstain with a nuclear dye to distinguish compartments.
• For ELISA, pair this antibody with a suitable detection antibody after testing dynamic range and matrix effects in your sample type (e.g., tissue homogenates, cell lysates).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering a comprehensive portfolio of antibodies, antigens, and ancillary reagents. This Arginase-1 polyclonal antibody supports research into urea cycle disorders, macrophage biology, and tumor immunology. In addition, CamelBio provides custom antibody development, bulk supply, and technical support to accelerate your assay development and regulatory submissions.