Anti-Arginase 1 (ARG1) Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P05089

Core Product Specifications and Parameters

Biological Background: Arginase-1 Function and Localization

• ARG1 is a key urea cycle enzyme in the liver, hydrolyzing L-arginine to urea and L-ornithine, which can be further metabolized to proline and polyamines that support collagen synthesis and cell proliferation.
• In nonhepatic tissues, ARG1 regulates L-arginine availability by competing with nitric oxide synthase (NOS), thereby modulating innate and adaptive immune responses.
• In polymorphonuclear granulocytes (PMNs), ARG1 participates in an antimicrobial effector pathway; upon PMN cell death, it is released and depletes arginine in the microenvironment, leading to suppressed T-cell and natural killer (NK) cell proliferation and cytokine secretion. Related references: PMID:15546957 PMID:16709924 PMID:19380772
• ARG1 promotes acute type 2 inflammation in the lung and supports optimal proliferation of group 2 innate lymphoid cells (ILC2s).
• Localized to the cytoplasm and cytoplasmic granules, ARG1 contains a manganese cofactor essential for its catalytic activity.
• ARG1 is predominantly expressed in the liver, with additional immune expression in granulocytes, macrophages within mycobacterial granulomas, and ILC2s. Related references: PMID:15546957 PMID:23749634 PMID:27043409

Experimental Guidance and Technical Tips

• For Western blot detection, a band corresponding to ~35 kDa may be observed; consider using whole cell lysates from liver tissues or immune cells, and validate the antibody in your specific sample system.
• Immunofluorescence/immunoperoxidase (IF-P) experiments may require optimization of fixation and permeabilization conditions due to the cytoplasmic localization of ARG1.
• The antibody is suitable for ELISA applications; ensure appropriate antigen coating and blocking steps are optimized for your assay.
• ARG1 expression can be induced under certain immunological conditions; consider using appropriate positive and negative controls from relevant human, mouse, or rat samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-ARG1 rabbit polyclonal antibody supports research into urea cycle metabolism and immune regulation, and CamelBio can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. For procurement assistance or technical inquiries, our team is ready to help accelerate your diagnostic development.