Anti-ARFRP1 Rabbit Polyclonal Antibody for WB and ELISA - Q13795

Core Product Specifications and Parameters

Biological Background: ARFRP1 Function and Localization

• ARFRP1 (ADP-ribosylation factor-related protein 1), also known as ARP or ARL18, is a small GTPase of the ARF family involved in intracellular membrane trafficking.
• It functions as a trans-Golgi network (TGN)-localized GTPase that critically regulates protein sorting, specifically controlling the targeting of ARL1 and its effectors to the TGN.
• ARFRP1 is required for the lipidation of chylomicrons in the intestine and the lipidation of very-low-density lipoproteins (VLDL) in the liver, highlighting its role in lipid metabolism.
• The protein is primarily localized to the Golgi apparatus and trans-Golgi network, consistent with its role in post-Golgi trafficking.
• Human ARFRP1 is expressed ubiquitously, being found in most tissues, which aligns with its fundamental cellular function.
• Post-translational modifications include acetylation, and the gene undergoes alternative splicing, potentially generating multiple isoforms.
• Molecular weight is approximately 23 kDa (predicted 22.6 kDa from sequence), and the protein contains conserved GTP-binding and nucleotide-binding domains typical of the Ras superfamily.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fragment covering the N-terminal amino acids 1-201 of human ARFRP1, which includes essentially the full-length protein. This design may enhance detection of multiple isoforms and denatured/reduced forms in Western blot.
• For Western blot, consider titrating the antibody concentration, as ARFRP1 is a low-abundance Golgi-associated protein. A starting dilution of 1:500–1:2000 is typical for polyclonal antibodies, but optimization is recommended.
• In ELISA applications, the antibody may be used for either direct antigen detection or as a capture/detection pair with a suitable monoclonal antibody; validate compatibility with your specific assay format.
• Since ARFRP1 is conserved across human, mouse, and rat, this antibody can serve as a tool for comparative studies across these species. Validate cross-reactivity in your relevant sample types.
• To maintain stability, avoid repeated freeze-thaw cycles. Aliquot and store at -20°C or -80°C for long-term storage.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For researchers and diagnostic developers focusing on Golgi trafficking and lipid metabolism, we supply high-quality tools like this anti-ARFRP1 polyclonal antibody, complemented by tailored bulk-order and custom service options. Reach out to us to streamline procurement of rare and critical raw materials.