Anti-ARFGAP1 Rabbit Polyclonal Antibody for WB, ELISA - Q8N6T3

Core Product Specifications and Parameters

Biological Background: ARFGAP1 Function and Localization

• ARFGAP1 (ADP-ribosylation factor GTPase-activating protein 1, also known as ARF1 GAP) is a GTPase-activating protein that specifically inactivates the small GTPase ARF1 by promoting the hydrolysis of bound GTP to GDP.
• By deactivating ARF1, ARFGAP1 facilitates the dissociation of coat proteins (such as COPI) from Golgi-derived membranes and vesicles, a critical step for vesicle fusion with target compartments.
• ARFGAP1 is implicated in membrane trafficking and vesicle transport, and its overexpression leads to the redistribution of the entire Golgi complex to the endoplasmic reticulum, mimicking ARF1 inactivation.
• It localizes to the cytoplasm and the Golgi apparatus, consistent with its role in ER-Golgi transport.
• The protein contains zinc-finger domains and is regulated by phosphoinositides and phosphatidylcholine; it is subject to post-translational modifications including acetylation and phosphorylation.
• Alternative splicing of ARFGAP1 generates multiple isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• Recommend using whole cell lysates of human, mouse, or rat origin for Western blotting, as the antibody is cross-reactive.
• For ELISA, consider using recombinant ARFGAP1 protein or cell lysates as coating antigen.
• The immunogen encompasses the N-terminal region (aa 1-220), which may be conserved across species; thus, the antibody is expected to detect ARFGAP1 in indicated species.
• Note that ARFGAP1 has a predicted molecular weight of approximately 44.7 kDa, but post-translational modifications or isoforms may result in bands around 45 kDa or multiple bands.
• Consider validating the antibody in the relevant sample system where ARFGAP1-mediated vesicle trafficking is studied.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material partner, CamelBio provides diagnostic manufacturers and researchers with one-stop access to validated antibodies, bulk ancillary reagents, and specialty raw materials. This anti-ARFGAP1 rabbit polyclonal antibody supports investigations into membrane trafficking, Golgi dynamics, and ARF1 signaling pathways. Our comprehensive portfolio and sourcing capabilities help accelerate your assay development from concept to clinic.