Anti-ARF6 Polyclonal Antibody for WB, IF/ICC, ELISA - P62330

Core Product Specifications and Parameters

Biological Background: ARF6 Function and Localization

• ARF6 (ADP-ribosylation factor 6) is a small GTPase of the ARF family, encoded by the ARF6 gene, that cycles between GDP-bound inactive and GTP-bound active states to regulate multiple aspects of intracellular membrane trafficking.
• As a central player in endocytic recycling, ARF6 controls the return of internalized cargo from recycling endosomes to the plasma membrane, facilitating cytoskeleton remodeling and cell migration (PubMed:11266366, PubMed:16737952, PubMed:18400762).
  Related references: PMID:11266366 PMID:16737952 PMID:18400762
• In its GTP-bound form, ARF6 mediates the transport of palmitoylated proteins from the trans-Golgi network to the plasma membrane, a function critical for polarized secretion and cell surface receptor delivery (PubMed:37461827).
  Related references: PMID:37461827
• ARF6 is essential for mitotic cytokinesis, localizing to the cleavage furrow and midbody to ensure proper daughter cell separation.
• The protein actively modulates neuronal morphology by regulating dendritic spine development, branching, and filopodia extension, and potentiates neurite outgrowth through interaction with the adapter protein APBB1 (PubMed:14978216, PubMed:36250347).
  Related references: PMID:14978216 PMID:36250347
• During epithelial polarization, ARF6 governs junctional remodeling by controlling the surface levels of adherens junction proteins such as CDH1, and plays a role in NTRK1 recycling from early endosomes (PubMed:36017701).
  Related references: PMID:36017701
• ARF6 is also implicated in microbial pathogenesis: it allosterically activates cholera toxin and facilitates the entry of enterovirus 71 into brain endothelial cells.
• Ubiquitously expressed with higher levels in heart, substantia nigra, and kidney, ARF6 localizes to multiple membrane compartments including the plasma membrane, endosomes, Golgi, and is post-translationally modified by myristoylation.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1–175 of human ARF6, encompassing the N-terminal GTP-binding domain; the resulting polyclonal antibody may detect both GDP- and GTP-loaded conformations.
• Western blot analysis is expected to reveal a band at approximately 20 kDa; use lysates from human, mouse, or rat cell lines or tissues as positive controls.
• For immunofluorescence/ICC, ARF6 staining is anticipated at the plasma membrane, recycling endosomes, and intracellular vesicles; fixation and permeabilization conditions should be optimized for membrane-associated targets.
• ELISA applications can be developed using recombinant ARF6 protein or peptide fragments; consider including negative controls to assess background binding.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material supplier, CamelBio provides diagnostic developers and researchers with seamless access to validated antibodies and related reagents. This anti-ARF6 polyclonal antibody, targeting a key regulator of membrane trafficking and cytoskeleton dynamics, is one of many tools we offer to accelerate assay development in areas such as cell biology, neurology, and infectious disease. With robust supply chain capabilities, we can deliver bulk quantities of optimized antibodies, matched antigen pairs, and ancillary materials to support your project from concept to clinical application.