Anti-ARF6 Monoclonal Antibody for WB, ELISA - P62330

Core Product Specifications and Parameters

Biological Background: ARF6 Function and Localization

• ARF6 is a small GTP-binding protein that cycles between inactive GDP-bound and active GTP-bound states, regulating membrane trafficking and actin cytoskeleton dynamics.
• It plays a critical role in endocytic recycling, sorting cargo from early endosomes to the plasma membrane, including palmitoylated proteins (PubMed:37461827).
  Related references: PMID:37461827
• ARF6 is required for normal completion of mitotic cytokinesis, localizing to the cleavage furrow and midbody (By similarity).
• Involved in neuronal development: regulates dendritic spine development, dendritic branching, and filopodia extension (PubMed:14978216), and potentiates neurite outgrowth via interaction with APBB1 (PubMed:36250347).
  Related references: PMID:14978216 PMID:36250347
• Functions in epithelial polarization and junctional remodeling by regulating surface levels of adherens junction proteins such as CDH1 (PubMed:36017701).
  Related references: PMID:36017701
• During microbial infection, ARF6 acts as an allosteric activator of cholera toxin catalytic subunit and facilitates endocytosis of enterovirus 71 for viral entry into brain endothelial cells (PubMed:11266366, PubMed:16737952, PubMed:18400762).
  Related references: PMID:11266366 PMID:16737952 PMID:18400762
• Subcellular localization includes cytoplasm, cell membrane, endosome membranes (especially recycling endosome), Golgi, filopodia, ruffles, and midbody. It is ubiquitously expressed with higher levels in heart, substantia nigra, and kidney.
• Keywords: GTP-binding, lipoprotein, myristate, protein transport, neurogenesis, cell division.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 76–175) of human ARF6, which shows high sequence identity across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot, a band around 20 kDa is expected; consider using reducing conditions and appropriate loading controls.
• For ELISA, immobilize antigen or antibody according to standard protocols; specificity should be validated with recombinant ARF6 protein or cell lysates.
• As ARF6 is membrane-associated and may be palmitoylated, consider subcellular fractionation to enrich for membrane fractions if detection is weak in whole-cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing service for IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this anti-ARF6 clone. Whether you need bulk ancillary reagents or rare target raw materials for research on endocytic recycling, cytoskeleton dynamics, neurobiology, or infectious diseases, we supply diagnostic manufacturers, labs, and research institutes with the tools to advance from concept to clinic.