Anti-ARF5 Rabbit Polyclonal Antibody for WB, ELISA - P84085

Core Product Specifications and Parameters

Biological Background: ARF5 Function and Localization

• ARF5 is a member of the ADP-ribosylation factor (ARF) family of small GTPases, which play essential roles in intracellular vesicular trafficking and organelle structure.
• It functions as a GTP-binding protein that contributes to vesicle budding and uncoating at the Golgi apparatus, mediating protein transport between the endoplasmic reticulum and Golgi.
• ARF5 regulates mTORC1 activity by promoting the recruitment of mTORC1 to plasma membrane ruffles, thereby facilitating its activation via the PI3K/Akt signaling pathway. Related references: PMID:36735494
• It acts as an early responder to lysosomal damage and participates in lysosomal quality control pathways, highlighting its role in maintaining cellular homeostasis. Related references: PMID:41293316
• During microbial infection, ARF5 serves as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase, indicating its involvement in host-pathogen interactions.
• The protein localizes to multiple cellular compartments, including the cell membrane, Golgi apparatus, trans-Golgi network membrane, cytoplasm (perinuclear region), and lysosome membrane.
• ARF5 is characterized by post-translational modifications such as N-myristoylation, which anchors it to membranes, and it contains conserved GTP-binding domains essential for its regulatory functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-180) of human ARF5. This region includes the conserved GTP-binding domain and is likely to detect full-length ARF5. Consider using a positive control lysate from HeLa, HEK293, or other human cell lines expressing ARF5 for Western blot validation.
• For Western blot, the predicted molecular weight of ARF5 is approximately 21 kDa. Use reducing SDS-PAGE conditions and a standard blocking buffer. Optimize primary antibody dilution empirically between 1:500 and 1:2000, and include a species-appropriate secondary antibody.
• The antibody is suitable for ELISA applications. For direct or sandwich ELISA, titrate the antibody against the recombinant protein used as immunogen or against cell lysates to determine optimal coating and detection concentrations.
• Cross-reactivity is reported with human, mouse, and rat ARF5. When working with mouse or rat samples, validate the antibody in your specific model system, as post-translational modifications or protein expression levels may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, including this anti-ARF5 polyclonal antibody tailored for protein trafficking and mTOR signaling studies. As a one-stop sourcing partner, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—ensuring seamless integration from early research to clinical application. Let CamelBio streamline your raw material procurement so you can focus on advancing your assays.