Anti-ARF5 Monoclonal Antibody for WB, IF/ICC, ELISA - P84085

Core Product Specifications and Parameters

Biological Background: ARF5 Function and Localization

• ADP-ribosylation factor 5 (ARF5) is a small GTP-binding protein that cycles between active GTP-bound and inactive GDP-bound states, functioning as a molecular switch in intracellular trafficking.
• ARF5 plays a key role in protein trafficking, contributing to vesicle budding and uncoating at the Golgi apparatus.
• It regulates mTORC1 activity by promoting recruitment of the mTORC1 complex to plasma membrane ruffles, facilitating activation via the PI3K/Akt signaling pathway. Related references: PMID:36735494
• ARF5 acts as an early responder to lysosomal damage and participates in lysosomal quality control pathways. Related references: PMID:41293316
• In microbial infection, ARF5 functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
• ARF5 is localized to multiple cellular compartments including the Golgi apparatus (trans-Golgi network membrane), cell membrane, cytoplasm (perinuclear region), and lysosome membrane, reflecting its dynamic trafficking roles.
• It is a myristoylated lipoprotein, with a myristoyl group attached to its N-terminus, which likely facilitates membrane association.
• The protein has a calculated molecular weight of approximately 20.5 kDa and belongs to the ARF family of small GTPases.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 81–180 of human ARF5, which is conserved across human, mouse, and rat; therefore, the antibody may detect ARF5 in these species, but cross-reactivity should be validated in the relevant experimental system.
• For Western blot (WB), ARF5 is predicted at ~21 kDa; consider using appropriate positive controls (e.g., HeLa, HEK293 cell lysates) and confirm band specificity with siRNA or knockout validation.
• In immunofluorescence (IF/ICC), ARF5 localizes to the Golgi and cytoplasmic vesicles; fixation and permeabilization conditions should be optimized for Golgi markers, and co-staining with organelle markers is recommended.
• For ELISA, the antibody can be used as a detection or capture reagent; pairing with a suitable ARF5 antibody pair may be necessary for sandwich ELISA setups.
• Since ARF5 is a myristoylated membrane-associated protein, extraction buffers containing mild detergents may improve solubility without disrupting epitope recognition.

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CamelBio provides diagnostic manufacturers and research institutions with streamlined access to high-quality IVD raw materials, including this Anti-ARF5 Rabbit Monoclonal Antibody. Beyond antibodies, we offer a comprehensive portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your intracellular trafficking and signal transduction research applications. Our one-stop service model covers every stage from concept to clinic, ensuring reliable and efficient procurement for your diagnostic development needs.