Anti-ARF5 (KO Validated) Rabbit Polyclonal Antibody for WB, ELISA - P84085

Core Product Specifications and Parameters

Biological Background: ARF5 Function and Localization

• ARF5 is a member of the ADP-ribosylation factor (ARF) family of small GTPases, which play crucial roles in vesicular trafficking and organelle dynamics.
• It contributes to vesicle budding and uncoating at the Golgi apparatus, facilitating intracellular protein transport.
• ARF5 regulates mTORC1 activity at the plasma membrane by promoting its recruitment to membrane ruffles and enabling activation via the PI3K/Akt pathway. Related references: PMID:36735494
• It acts as an early responder to lysosomal damage and participates in lysosomal quality control pathways. Related references: PMID:41293316
• Additionally, during microbial infection, ARF5 functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
• Subcellularly, ARF5 localizes to the cell membrane, Golgi apparatus (including the trans-Golgi network), cytoplasm, perinuclear region, and lysosome membrane.
• ARF5 is a lipoprotein modified by N-terminal myristoylation, a feature essential for its membrane association and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-180 of human ARF5, suggesting the antibody may recognize the N-terminal region of the protein.
• For Western blot, ARF5 is predicted to migrate at approximately 21 kDa; consider using appropriate molecular weight markers and loading controls.
• Knockout validation (as indicated) can help confirm antibody specificity in relevant cell lines or tissue samples.
• ELISA can be employed for quantitative detection; optimization of coating conditions and antibody concentration is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering a comprehensive portfolio that includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents. This ARF5 antibody supports research in Golgi trafficking and mTORC1 signaling pathways. Leverage our sourcing expertise to accelerate your diagnostic development from concept to clinic.