Anti-ARF4 Polyclonal Antibody for WB, IF/ICC, ELISA - P18085

Core Product Specifications and Parameters

Biological Background: ARF4 Function and Localization

• ARF4 (ADP-ribosylation factor 4, also known as ARF2) is a low-molecular-weight GTP-binding protein belonging to the ADP-ribosylation factor family.
• It functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase, and is involved in intracellular protein trafficking.
• ARF4 plays a role in modulating vesicle budding and uncoating within the Golgi apparatus, contributing to ER-Golgi transport.
• It is a critical component of the ciliary targeting complex, interacting with Rab11, ASAP1, Rabin8/RAB3IP, and RAB11FIP3 to direct preciliary vesicle trafficking to the mother centriole and initiate ciliogenesis. Related references: PMID:25673879
• The protein localizes primarily to the Golgi apparatus and is membrane-associated.
• ARF4 undergoes post-translational modifications such as myristoylation and phosphorylation, as indicated by UniProt keywords.
• It has been identified in large-scale proteomics studies and is part of the human reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-180 (N-terminal region) of human ARF4; verify antibody specificity in your sample system using appropriate negative controls.
• For Western blot (WB), the predicted molecular weight is approximately 21 kDa; optimize gel percentage and transfer conditions accordingly.
• In immunofluorescence/immunocytochemistry (IF/ICC), expect Golgi-like perinuclear staining; include organelle markers to confirm localization.
• For ELISA applications, follow standard direct or indirect protocols and consider using the recombinant immunogen as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with a one-stop supply of IVD raw materials, including validated anti-ARF4 polyclonal antibodies for protein trafficking and ciliogenesis studies. Our portfolio extends to customized antibody development, bulk ancillary reagents, and rare target sourcing, empowering your assay development from concept to clinic. Let CamelBio streamline your procurement with reliable, research-grade reagents for ciliary biology and beyond.