Anti-ARF1 Rabbit Polyclonal Antibody for WB, ELISA - P84077

Core Product Specifications and Parameters

Biological Background: ARF1 Function and Localization

• ARF1 (ADP-ribosylation factor 1) is a small GTPase of the ARF family, encoded by the ARF1 gene in humans.
• It regulates protein trafficking by modulating vesicle budding and uncoating within the Golgi complex. Related references: PMID:8253837
• In the GTP-bound state, ARF1 recruits coatomer proteins to Golgi membranes; GTP hydrolysis by ARFGAPs triggers coat dissociation, a step essential for transport vesicle formation. Related references: PMID:8253837
• ARF1 interacts with PICK1 to limit inhibition of the Arp2/3 complex, thereby modulating AMPA receptor trafficking and spine shrinkage during long-term depression.
• It maintains intestinal homeostasis by regulating stem cells and gut microbiota, and promotes mast cell expansion through mTORC1 activation.
• During microbial infection, ARF1 acts as an allosteric activator of cholera toxin’s catalytic subunit.
• ARF1 is predominantly localized at the Golgi apparatus membrane, and also found at synapses, synaptosomes, and postsynaptic densities.
• Post-translational modifications include N-myristoylation and acetylation; it features GTP-binding and hydrolase activities.

Experimental Guidance and Technical Tips

• The immunogen covers full-length ARF1 (aa 1‑181), which may recognize both GTP- and GDP-bound conformations; consider using nucleotide-specific conditions for functional studies.
• For Western blot, expect a band at approximately 21 kDa. Validate in the relevant sample system and include proper controls to confirm specificity.
• Cross-reactivity with mouse and rat supports rodent model experiments; however, tissue-specific expression levels should be verified.
• In ELISA applications, use recombinant ARF1 as a standard and optimize coating conditions for reproducible results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—covering every stage from concept to clinic. This anti-ARF1 polyclonal antibody supports research in intracellular trafficking, synaptic plasticity, and host‑pathogen interactions. Contact CamelBio for reliable supply and technical assistance to accelerate your diagnostic assay development.