Anti-ARF1 Polyclonal Antibody for WB, ELISA - P84077

Core Product Specifications and Parameters

Biological Background: ARF1 Function and Localization

• ARF1 is a small GTPase of the ARF family, acting as a binary switch in membrane trafficking pathways.
  Related references: PMID:8253837

• It regulates vesicle budding/uncoating in the Golgi by recruiting coatomer in its GTP-bound form.
  Related references: PMID:8253837

• ARF1-bound GTP hydrolysis by ARFGAP proteins triggers coat dissociation, a critical step for vesicle maturation and cargo delivery.
  Related references: PMID:8253837

• ARF1 limits PICK1-mediated inhibition of Arp2/3, thereby modulating AMPA receptor trafficking and synaptic plasticity (LTD). (By similarity)

• It maintains intestinal homeostasis by regulating stem cells and gut microbiota. (By similarity)

• ARF1 facilitates mast cell expansion via optimal mTORC1 activation. (By similarity)

• It acts as an allosteric activator of cholera toxin ADP-ribosyltransferase during infection.

• ARF1 localizes to the Golgi membrane, synaptic compartments, and postsynaptic density; post-translational modifications include acetylation, and the gene is linked to the disease variant PVNH8.

Experimental Guidance and Technical Tips

• Because the immunogen is a synthetic peptide from amino acids 100–181, the antibody may recognize linear epitopes within this central region; consider peptide blocking experiments to confirm specificity.
• For Western blot, expect a band near 21 kDa in human, mouse, and rat samples; validate in the relevant tissue/cell line expressing ARF1.
• In ELISA, the antibody can be used as a capture or detection reagent; optimize coating and detection conditions for the target application.
• Given the polyclonal nature, lot-to-lot consistency should be confirmed for critical quantitative analyses.
• For immunofluorescence or other applications not listed, perform validation as sensitivity and cross-reactivity may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and research labs, from discovery to clinical stages. This anti-ARF1 antibody supports studies in Golgi trafficking, synaptic plasticity, and host-pathogen interactions. Beyond this product, CamelBio offers validated antibody pairs, optimized recombinant antibodies, and ancillary reagents for assay development, helping you accelerate your biomarker and therapeutic programs.