Anti-ARCN1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P48444

Core Product Specifications and Parameters

Biological Background: ARCN1 Function and Localization

• ARCN1 encodes coatomer subunit delta, also known as archain or delta-COP, a component of the coatomer (COPI) complex.
• The coatomer complex is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi-derived non-clathrin-coated vesicles, mediating biosynthetic protein transport from the ER through the Golgi.
• Required for budding from Golgi membranes and essential for retrograde Golgi-to-ER transport of dilysine-tagged proteins.
• Recruitment to membranes is mediated by ADP-ribosylation factors (ARFs), small GTP-binding proteins.
• Influences Golgi structural integrity and also plays a role in the processing, activity, and endocytic recycling of LDL receptors (By similarity).
• Localizes to the cytoplasm, Golgi apparatus membrane, and COPI-coated vesicle membrane.
• Ubiquitously expressed in tissues.
• Post-translational modifications include acetylation and phosphorylation; undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen sequence spans amino acids 212–511 of human ARCN1, a region conserved in mouse and rat, consistent with reported cross-reactivity.
• For Western blotting, a band corresponding to approximately 57 kDa is expected; optimize loading and transfer conditions.
• In immunofluorescence/ICC, ARCN1 localizes to the Golgi apparatus; appropriate fixation and permeabilization may enhance detection.
• ELISA protocols: consider antigen coating concentration and antibody titration for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with comprehensive IVD raw material solutions. For cytoskeletal and trafficking targets like ARCN1, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk reagent supply to streamline your assay development. Whether you need rare target sourcing or ancillary reagents, CamelBio supports your project from concept to clinic.