Anti-ARC (hArc/Arg3.1) Rabbit Polyclonal Antibody for WB, ELISA - Q7LC44

Core Product Specifications and Parameters

Biological Background: ARC Function and Localization

• ARC (Activity-regulated cytoskeleton-associated protein), also known as Arg3.1, is a master regulator of synaptic plasticity that self-assembles into virion-like capsids capable of encapsulating and transferring RNAs between neurons.
• ARC is critically required for protein synthesis-dependent long-term potentiation (LTP) and long-term depression (LTD), as well as for the consolidation of long-term memory.
• It promotes endocytosis of AMPA receptors (AMPARs) in response to synaptic activity, playing a key role in synaptic scaling and neuronal homeostasis.
• ARC accumulates at weaker synapses and mediates activity-dependent synapse elimination, contributing to the pruning of surplus climbing fiber synapses in the developing cerebellum.
• Subcellularly, ARC localizes to postsynaptic densities, dendritic spines, early endosomes, clathrin-coated vesicles, the cell cortex, and is also found in extracellular vesicles.
• Post-translational modifications include palmitoylation, phosphorylation, and ubiquitination, which regulate its trafficking and function.
• Beyond the nervous system, ARC is expressed in skin-migratory dendritic cells and influences fast dendritic cell migration and T-cell activation.
• The human ARC gene encodes a protein of approximately 45 kDa, and its expression is rapidly induced by neuronal activity.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1–220) of human ARC; the polyclonal nature may recognize multiple epitopes, enhancing sensitivity in Western blot.
• For Western blot, a band at ~45 kDa is expected in neuronal lysates; consider using brain tissue or cultured neuron extracts as positive controls.
• Cross-reactivity with mouse makes this antibody suitable for rodent model studies; validate in the relevant sample system to confirm specificity.
• In ELISA, the antibody can be used with recombinant ARC protein; optimize coating and detection conditions for best signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this high-quality anti-ARC polyclonal antibody, supporting neuroscience and synaptic plasticity research with reliable raw materials. Whether you need validated antibody pairs, optimized monoclonal or polyclonal reagents, or bulk ancillary reagents, we streamline procurement for diagnostic and laboratory workflows. As a one-stop IVD partner, CamelBio bridges your concept-to-clinic journey, offering one-stop access to rare-target raw materials like this ARC antibody alongside comprehensive technical support.