Anti-ARAF Polyclonal Antibody for WB, IF/ICC, ELISA - P10398

Core Product Specifications and Parameters

Biological Background: ARAF Function and Localization

• Serine/threonine-protein kinase that belongs to the RAF family of proto-oncogenes. Encoded by the ARAF gene, with alternative splicing producing multiple isoforms.
• Key component of the RAS-RAF-MEK-ERK signal transduction pathway, relaying mitogenic signals from the cell membrane to the nucleus to regulate cell proliferation and differentiation.
• Also implicated in the TOR signaling cascade, linking growth factor signals to cellular metabolism and growth.
• Phosphorylates PFKFB2, a bifunctional enzyme that controls glycolysis, providing a direct link between ARAF activity and metabolic regulation. Related references: PMID:36402789
• Functions as a positive regulator of myogenic differentiation by inducing cell cycle arrest, upregulating myogenin and other muscle-specific proteins, and promoting myotube formation.
• Predominantly expressed in urogenital tissues, suggesting a specialized physiological role in these organs.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 371–606) of human ARAF. This region is relatively conserved, so the antibody may recognize all known isoforms and cross-react with mouse and rat ARAF as indicated.
• For Western blot (WB), the predicted molecular weight is approximately 68 kDa. Use appropriate gel percentage and transfer conditions for this size range.
• For immunofluorescence/immunocytochemistry (IF/ICC), consider optimizing fixation and permeabilization protocols, as detection may depend on epitope accessibility in different cellular compartments.
• The antibody is also suitable for ELISA; consider pairing it with a suitable matched antibody pair or using it for direct antigen detection assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic manufacturers and research laboratories by providing a single access point for high-quality IVD raw materials. This anti-ARAF polyclonal antibody supports key research areas in kinase signaling and muscle differentiation. Beyond individual reagents, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Let CamelBio be your trusted partner from concept to clinic.