Anti-Aquaporin-4 (AQP4) Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P55087

Core Product Specifications and Parameters

Biological Background: Aquaporin-4 (AQP4) Function and Localization

• Aquaporin-4 (AQP4), also known as Mercurial-insensitive water channel (MIWC) and WCH4, is a member of the aquaporin family of integral membrane proteins that facilitate water transport across cell membranes.
• It forms a water-specific channel (PubMed:19383790, PubMed:7559426, PubMed:8601457) and plays an essential role in brain water homeostasis (PubMed:37143309). AQP4 is integral to glymphatic solute transport, mediating the clearance of soluble beta-amyloid peptides from the brain interstitial fluid. Related references: PMID:19383790, PMID:7559426, PMID:8601457, PMID:37143309
• Subcellularly, AQP4 localizes predominantly to the cell membrane, including basolateral membranes in epithelia, and is also found in endosome membranes, the sarcolemma, and cellular projections such as astrocyte end-feet.
• It is expressed in several tissues, with high levels in brain, lung, and skeletal muscle, moderate levels in stomach (fundic gland), and lower levels in heart and ovary.
• Post-translational modifications of AQP4 include palmitoylation, phosphorylation, and glycosylation, which may influence its trafficking, stability, and channel activity.
• AQP4 plays a redundant role in urinary water homeostasis and urinary concentrating ability (by similarity), though its primary function is in the central nervous system.

Experimental Guidance and Technical Tips

• The immunogen (aa 256–323) corresponds to the intracellular C-terminal domain of human AQP4, which is highly conserved across human, mouse, and rat (as indicated by the reported cross-reactivity). Researchers can leverage this to detect AQP4 across these species.
• For western blot (WB), the predicted molecular weight is ~35 kDa. Note that post-translational modifications (e.g., glycosylation, palmitoylation) may result in slightly higher apparent molecular weights; validation in the relevant sample system is recommended.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), AQP4’s localization to basolateral membranes and cellular projections should be considered. Optimize permeabilization and antigen retrieval protocols to ensure accurate detection.
• For ELISA, the antibody can be used to capture or detect AQP4; however, users should validate the antibody pair and assay conditions in their specific matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers and research laboratories with reliable access to antibodies, antigens, and ancillary reagents. Our Aquaporin-4 polyclonal antibody supports studies into brain water homeostasis and glymphatic clearance—key areas in neurological research. Beyond this product, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk raw materials, and assistance in sourcing rare-target reagents to help accelerate your diagnostic development from concept to clinic.