Anti-Aquaporin-4 (AQP4) Rabbit Monoclonal Antibody for WB - P55087

Core Product Specifications and Parameters

Biological Background: Aquaporin-4 Function and Localization

• Aquaporin-4 (AQP4), also known as mercurial-insensitive water channel (MIWC) or WCH4, is a transmembrane water channel protein that facilitates water transport across cell membranes (PubMed:19383790, PubMed:7559426, PubMed:8601457). Related references: PMID:19383790 PMID:7559426 PMID:8601457
• AQP4 plays a critical role in brain water homeostasis and is essential for glymphatic solute transport, regulating cerebrospinal fluid influx into the brain parenchyma and drainage of interstitial fluid, thereby clearing solutes like amyloid-beta peptides (PubMed:37143309). Related references: PMID:37143309
• It has a redundant role in urinary water homeostasis and urinary concentrating ability (By similarity).
• The protein localizes to the cell membrane, basolateral cell membrane, endosome membrane, sarcolemma, and cell projections, reflecting its diverse roles in water homeostasis in various tissues.
• AQP4 is expressed in brain, lung, skeletal muscle, stomach, heart, and ovary, with high levels in brain and skeletal muscle (PubMed:7559426, PubMed:8601457, PubMed:29055082). Related references: PMID:7559426 PMID:8601457 PMID:29055082
• Post-translational modifications include palmitoylation and phosphorylation, and it is predicted to be glycosylated; these may regulate its localization and function.
• Known isoforms generated by alternative splicing may have distinct tissue distributions and functional properties.

Experimental Guidance and Technical Tips

• The immunogen for this antibody corresponds to amino acids 256-323 of human AQP4 (NP_001641.1), a region in the cytoplasmic C-terminal domain. This region may be conserved across isoforms, potentially enabling detection of multiple splice variants.
• For Western blotting (WB), expect bands around 32-34 kDa; however, glycosylation and other modifications may result in additional bands. Validate in your specific sample system.
• For immunohistochemistry (IHC) and immunofluorescence (IF), confirm antibody specificity using appropriate controls, especially in tissues with high AQP4 expression like brain and kidney.
• This antibody is suitable for ELISA; consider optimizing coating antigen and detection conditions using recombinant AQP4 protein.
• Cross-reactivity is reported for human, mouse, and rat. For other species, verify sequence homology before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories as a comprehensive supplier of IVD raw materials. For neuroscience and water homeostasis research, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents against AQP4 and other aquaporins. Our one-stop sourcing service covers rare target raw-material procurement, technical consultation, and seamless supply chain management, helping you accelerate from concept to clinical application.