Anti-Aquaporin-3 (AQP3) Rabbit Polyclonal Antibody for IHC-P, ELISA - Q92482

Core Product Specifications and Parameters

Biological Background: Aquaporin-3 (AQP3) Function and Localization

• Aquaporin-3 (AQP3), also known as aquaglyceroporin-3, is encoded by the AQP3 gene in humans and functions as a homotetrameric transmembrane channel where each monomer independently facilitates glycerol and water transport across the plasma membrane down their osmotic gradients.
  Related references: PMID:12239222 PMID:30420639
• AQP3 may also be permeable to urea and contributes to cellular permeability to hydrogen peroxide (H2O2), thereby participating in H2O2-mediated signaling pathways.
  Related references: PMID:20724658
• In the skin, AQP3 transports glycerol to the epidermis and stratum corneum, where it helps maintain hydration, elasticity, and supports lipid biosynthesis necessary for barrier repair.
• In the kidney, AQP3 participates in water reabsorption, contributing to the maintenance of proper fluid balance.
• At the subcellular level, AQP3 localizes to the cell membrane and specifically to the basolateral membrane in epithelial cells.
• AQP3 is widely expressed in epithelial cells of the kidney (collecting ducts) and airways, as well as in keratinocytes, immature dendritic cells, and erythrocytes. An alternatively spliced isoform (isoform 2) is not detectable in erythrocytes at the protein level.
• Key annotations include alternative splicing, blood group antigen, glycoprotein, and transport, indicating post-translational modifications such as glycosylation and a role as a membrane transport protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide spanning amino acids 193–292 of human AQP3 (NP_004916.1), targeting the C-terminal region; users may consider this region for detecting total AQP3 in their assays.
• For immunohistochemistry (IHC-P), perform appropriate antigen retrieval and validate using known positive tissue controls such as kidney or skin, where AQP3 is abundantly expressed.
• For ELISA, the antibody may be suitable for both direct antigen capture and sandwich formats; it is recommended to optimize coating conditions and antibody dilutions with the relevant sample system.
• The predicted molecular weight is approximately 32 kDa; however, note that glycosylation (suggested by “Glycoprotein” keyword) may cause a shift in observed molecular weight on SDS-PAGE, so additional validation with deglycosylation enzymes may be considered if exact size confirmation is required.

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