Anti-Aquaporin-10 (AQP10) Rabbit Polyclonal Antibody for WB and ELISA - Q96PS8

Core Product Specifications and Parameters

Biological Background: Aquaporin-10 Function and Localization

• Aquaporin-10 (AQP10) is an aquaglyceroporin that forms homotetrameric transmembrane channels primarily responsible for glycerol and water transport across the plasma membrane following their osmotic gradients (PubMed:11573934, PubMed:12084581, PubMed:21733844). Related references: PMID:11573934 PMID:12084581 PMID:21733844
• It displays a unique pH-gated glycerol transport activity, being more active at acidic pH, and is likely involved in glycerol efflux from adipocytes during lipolysis and glycerol uptake by enterocytes (PubMed:11573934, PubMed:23382902, PubMed:30420639). Related references: PMID:11573934 PMID:23382902 PMID:30420639
• AQP10 can also transport urea, though with lower affinity compared to glycerol (PubMed:12084581). Related references: PMID:12084581
• The protein localizes to the apical cell membrane and lipid droplets, consistent with a role in vectorial glycerol transport in polarized epithelia.
• Tissue expression: Highest in absorptive epithelial cells at the tips of villi in the jejunum, and also detected in ileum, stomach, colon, rectum, and white adipose tissue (PubMed:11573934, PubMed:12084581, PubMed:15221416, PubMed:23382902). Related references: PMID:11573934 PMID:12084581 PMID:15221416 PMID:23382902
• AQP10 is a glycoprotein with several transmembrane helices; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 209-301, corresponding to a C-terminal intracellular region; thus, the antibody is predicted to recognize the C-terminal domain of AQP10.
• For Western blot, a band around 32 kDa is expected, but glycosylation may cause higher molecular weight species; validate with deglycosylation if needed.
• Cross-reactivity is reported for human and mouse; test in relevant tissue lysates (e.g., jejunum, adipose tissue).
• As a polyclonal antibody, lot-to-lot variation may occur; consider titration and proper controls for each application.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we bridge the gap between raw-material supply and IVD development by offering a comprehensive portfolio of antibodies, antigens, and ancillary reagents. For aquaporin-10 research and assay development, this anti-AQP10 polyclonal antibody serves as a vital tool for studying glycerol transport and metabolic disorders, and we can assist with bulk orders, matched antibody pairs, and customized solutions to accelerate your project from concept to clinic.