Anti-Aquaporin-1 (AQP1) Rabbit Polyclonal Antibody for WB, IF, IHC, ELISA - P29972

Core Product Specifications and Parameters

Biological Background: Aquaporin-1 Function and Localization

• Aquaporin-1 (AQP1), also known as CHIP28 or Aquaporin-CHIP, is a channel-like integral membrane protein of 28 kDa that forms a water-selective channel facilitating rapid water transport across cell membranes.
• It plays a central role in water homeostasis in various tissues, including renal concentrating mechanisms and epithelial fluid transport. Related references: PMID:1373524 PMID:23219802
• Additionally, AQP1 may be permeable to small solutes such as hydrogen peroxide, glycerol, ammonia, nitric oxide, and carbon dioxide, suggesting roles in gas and solute transport. Related references: PMID:16682607 PMID:17012249 PMID:19273840
• Recruited to the ankyrin-1 complex in erythrocytes, AQP1 is proposed to form a CO2 metabolon, linking CO2 diffusion, anion exchange, and carbonic acid interconversion. Related references: PMID:17012249 PMID:35835865
• In vitro studies indicate that AQP1 exhibits non-selective cation channel activity, potentially allowing passage of K+ and Na+ under certain conditions. Related references: PMID:36949749 PMID:8703053
• AQP1 localizes primarily to the cell membrane, consistent with its function as a transmembrane water channel.
• It is expressed in erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney, and pancreas, with lower levels in brain, placenta, and liver.
• Keywords include alternative splicing, blood group antigen, glycoprotein, phosphoprotein, transport, and transmembrane helix, reflecting its diverse regulatory and functional features.

Experimental Guidance and Technical Tips

• The immunogen spans a sequence (aa 200-269) within the intracellular region of human AQP1; consider this topology when designing experiments requiring antibody access in non-permeabilized specimens.
• For Western blot, a major band near 29 kDa is expected; glycosylated or complexed forms may migrate at higher molecular weights.
• Cross-reactivity with mouse and rat has been reported; validation in the specific sample matrix is recommended.
• This polyclonal antibody has been used in WB, IF-P, IHC-P, and ELISA; for immunohistochemistry, antigen retrieval conditions may need optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in securing rare-target raw materials. This AQP1 antibody supports research into water channels and renal function; CamelBio can help identify complementary reagents and customized solutions tailored to your diagnostic development needs.