Anti-Aquaporin-1 (AQP1) Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P29972

Core Product Specifications and Parameters

Biological Background: Aquaporin-1 Function and Localization

• Aquaporin-1 (AQP1; also known as CHIP28) is a water channel protein that facilitates rapid water transport across the plasma membrane in response to osmotic gradients, playing a key role in water homeostasis across various tissues including kidney, erythrocytes, and lung. Related references: PMID:1373524, PMID:23219802
• AQP1 belongs to the aquaporin family of integral membrane proteins and is a major component of the erythrocyte membrane, where it serves as the Colton blood group antigen and participates in a CO2 metabolon involving anion exchange and carbonic anhydrase. Related references: PMID:17012249, PMID:35835865
• Besides water, AQP1 may also allow passage of small solutes such as hydrogen peroxide, glycerol, and gases (NH3, NO, CO2), expanding its functional repertoire in cellular signaling and gas exchange. Related references: PMID:16682607, PMID:17012249, PMID:19273840
• Under specific conditions, AQP1 exhibits non-selective monovalent cation channel activity, indicating a capacity for ion permeation that may contribute to cell volume regulation. Related references: PMID:36949749, PMID:8703053
• Subcellularly, AQP1 is predominantly located on the cell membrane, where it forms homotetrameric complexes essential for its water channel function.
• Post-translational modifications include phosphorylation, which modulates channel activity and trafficking, and it is annotated with keywords such as Alternative splicing, Blood group antigen, Glycoprotein, and Transmembrane helix.
• Tissue expression is widespread, with strong detection in erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney, and pancreas, while brain, placenta, and liver exhibit weaker expression.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 170-269 of human AQP1 (P29972); this region is intracellular, thus the antibody is suitable for detecting total AQP1 levels.
• For Western blot (WB), consider using reducing/denaturing conditions and validate the expected band at ~29 kDa; note that glycosylation may cause band shifts in some samples.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), membrane staining should be observed; include appropriate permeabilization steps and validate signal specificity using knockout controls or blocking with immunogen peptide.
• For ELISA-based assays, use a standard indirect ELISA protocol and titrate both antibody and antigen to optimize signal-to-noise ratio.
• Cross-reactivity with mouse and rat AQP1 is reported, but sequence alignment between species should be checked for specific epitope conservation in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers an integrated sourcing solution for IVD manufacturers and research laboratories, providing validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents. This anti-AQP1 rabbit monoclonal antibody supports water channel and membrane transport studies, with CamelBio’s supply chain ensuring consistent lot-to-lot performance for diagnostic development. Additionally, we can source rare-target antibodies and custom formulations to meet your one-stop procurement needs from concept to clinic.