Anti-AQR Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O60306

Core Product Specifications and Parameters

Biological Background: RNA helicase aquarius Function and Localization

• RNA helicase aquarius (AQR), also known as intron-binding protein of 160 kDa (IBP160), is a spliceosomal protein involved in pre-mRNA splicing. Related references: PMID:11991638, PMID:25599396, PMID:28076346

• It acts as an intron-binding protein that links pre-mRNA splicing to small nucleolar ribonucleoprotein (snoRNP) biogenesis, facilitating position-dependent assembly of intron-encoded box C/D snoRNPs. Related references: PMID:16949364

• AQR possesses ATP-dependent RNA helicase activity, capable of unwinding double-stranded RNA molecules with a 3' overhang in vitro. Related references: PMID:25599396

• It is localized to the nucleus and nucleoplasm, consistent with its role in RNA processing.

• The protein belongs to the RNA helicase family and contains ATP-binding and RNA-binding domains essential for its function.

• Post-translational modifications include acetylation, which may regulate its activity and interactions within the spliceosome.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 400–700 of human AQR; consider this region when evaluating epitope conservation across species or isoforms.
• For Western blotting, the predicted molecular weight is approximately 171 kDa; optimize transfer conditions (e.g., longer transfer times, lower voltage) for high-molecular-weight proteins.
• In immunohistochemistry (paraffin), appropriate antigen retrieval methods should be validated in the target tissue to ensure optimal detection.
• ELISA can be employed for antibody titer determination or antigen detection; empirical optimization of coating concentration and blocking conditions is recommended.
• Cross-reactivity with mouse and rat AQR has been noted; verify sequence homology in the immunogen region if using these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research labs from initial development through to clinical application. This anti-AQR polyclonal antibody is a valuable tool for investigating spliceosome dynamics, RNA processing, and related diseases. Beyond single antibodies, we supply validated antibody pairs, bulk ancillary reagents, and can facilitate sourcing for rare or customized targets to accelerate your assay pipeline.