Anti-AQP5 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P55064

Core Product Specifications and Parameters

Biological Background: Aquaporin-5 Function and Localization

• Aquaporin-5 forms homotetrameric transmembrane channels, each monomer independently mediating water transport across the plasma membrane along its osmotic gradient. Related references: PMID:18768791 PMID:8621489
• Plays an important role in fluid secretion in salivary glands.
• Required for TRPV4 activation by hypotonicity and, together with TRPV4, controls regulatory volume decrease in salivary epithelial cells. Related references: PMID:16571723
• Localizes to the apical cell membrane, cell membrane, and cytoplasmic vesicle membrane.
• Detected in skin eccrine sweat glands, at the apical cell membrane and at intercellular canaliculi.
• Belongs to the aquaporin family and features six transmembrane helices with internal repeats; is subject to N-linked glycosylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a cytoplasmic region (residues 166–265); the antibody is suitable for detecting total AQP5 in WB and IF/ICC applications.
• For WB, expect a band near 28 kDa; consider using whole-cell lysates from salivary gland, lung, or skin tissues. Validate specificity with appropriate controls.
• In IF/ICC, fixation and permeabilization steps may be required to access the intracellular epitope; optimize for your cell or tissue type.
• Cross-reactivity with mouse and rat is indicated; verify in the relevant sample systems prior to routine use.
• The product is shipped on ice bags to maintain stability; upon receipt, store at recommended temperatures and avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials, including validated polyclonal and monoclonal antibodies, antibody pairs, and ancillary reagents. For aquaporin-related and fluid transport research, we offer curated anti-AQP5 reagents and supporting materials to accelerate assay development. From target validation to bulk supply, our one-stop platform bridges the gap from concept to clinic.