Anti-AQP5 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P55064

Core Product Specifications and Parameters

Biological Background: Aquaporin-5 Function and Localization

• AQP5 (Aquaporin-5, aliases PPKB, AQP-5) is a human aquaporin water channel encoded by the AQP5 gene (GeneID 362).
• Each AQP5 monomer functions independently within homotetrameric assemblies to mediate osmotically driven water transport across the plasma membrane (PubMed:18768791, PubMed:8621489). Related references: PMID:18768791, PMID:8621489
• AQP5 is essential for fluid secretion in salivary glands and, in cooperation with TRPV4, regulates cellular volume decrease upon hypotonic stress (PubMed:16571723). Related references: PMID:16571723
• The protein localizes predominantly to the apical plasma membrane and is also detected on cytoplasmic vesicle membranes.
• In skin, AQP5 is expressed in eccrine sweat glands, specifically at the apical membrane and intercellular canaliculi (at protein level).
• It is a glycoprotein with multiple transmembrane helices, characteristic of the aquaporin family.
• Clinically, AQP5 variants are implicated in palmoplantar keratoderma (keyword), highlighting its role in epidermal water homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within the C-terminal region (aa 165-265), which may recognize both glycosylated and unglycosylated forms; perform appropriate controls.
• For Western blotting, the predicted molecular weight is 28 kDa, but glycosylation may cause a shift; use a suitable blocking buffer.
• In immunohistochemistry (IHC-P), antigen retrieval conditions should be optimized, as the epitope is intracellular; permeabilization may enhance detection.
• Cross-reactivity with mouse and rat is specified, but validation in relevant tissues is recommended.
• For ELISA, consider using a specific coating buffer and ensure proper antigen presentation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a single point of access for all IVD raw materials, technical services, and consulting needs. For researchers studying aquaporin biology, epithelial water transport, or salivary gland function, CamelBio supplies validated antibody pairs, optimized monoclonal antibodies like this anti-AQP5 clone, and bulk ancillary reagents, along with support for rare-target raw-material sourcing to accelerate assay development from concept to clinic.